パルス電磁場刺激が骨芽細胞様細胞の増殖と分化に与える影響　免疫組織化学染色ならびに組織化学染色による検討

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  • Effect of Pulsing Electromagnetic Fields on the Proliferation and Differentiation of Osteoblast-like Cells. Studies on Using Immunohistochemical and Histochemical Staining Techniques.
  • Studies on Using Immunohistochemical and Histochemical Staining Techniques
  • 免疫組織化学染色ならびに組織化学染色による検討

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An immediate obtainment of osseointegration would shortened the initial anchorage period of implant within bone and setting time of the final superstructure. Application of pulsing electromagnetic fields (PEMFs) as a method for increasing the rate of osseointegration has been considered. PEMFs have been demonstrated to promote the proliferation and differentiation of osteoblast-like clone MC3T3-E1 cells.<BR>Recently, the immunohistochemical staining technique having Brd U or PCNA has been used as markers to study the proliferation dynamics of S-phase cells or late G, to S-phase cells. In this study, cell proliferation was examined in the experimental group having PEMFs-stimulation and in the control group lacking stimulation by immunohistochemical staining technique using Brd U and PCNA monoclonal antibodies. Brd Ulabeling and PCNA- positive indices were respectively calculated and compared. Further, alkaline phosphatase activity was investigated as a marker for cell differentiation of MC3T3-E1 cells by using a histochemical staining technique.<BR>The results suggested that the Brd U-labeling index and PCNA-labeling index distinctly increased in the experimental group (P<0.001). Cells in the S-phase and late G, to S-phases were 1.8 times and 1.6 times higher, respectively, than control. Alkaline phosphatase staining was stronger in the experimental group than in the control group.

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