韓国・済州島の馬とその飼育環境から分離された Rhodococcus equi の病原性プラスミド : 90-kb II型と新型90kb V型 Rhodococcus equi Virulence Plasmids Recovered from Horses and Their Environment in Jeju, Korea : 90-kb Type II and a New Variant, 90-kb Type V

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Author(s)

    • 高井 伸二 TAKAI Shinji
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 関 いづみ SEKI Izumi
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 山戸田 典子 YAMATODA Noriko
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 木村 亜弓 KIMURA Ayumi
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 木村 勤 KAKUDA Tsutomu
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 佐々木 由香子 SASAKI Yukako
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 椿 志郎 TSUBAKI Shiro
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • LIM Yoon-Kyu
    • Department of Veterinary Medicine, Cheju National University

Abstract

韓国・済州島の4カ所の済州馬の牧場と6カ所のサラブレッド種の牧場から採取した糞便と土壌よりRhodococcus equiを分離した.分離株における毒力関連15-17kDa抗原(VapA)とその遺伝子の存在を,モノクローナル抗体10G5を用いたコロニープロット法とPCR法で検索した.10カ所の牧場から採取した土壌36検体全てから1g当たり5.0×10^2〜7.5×10^4cfu,糞便40検体中37検体から1g当たり5.0×10^1〜1.1×10^5cfuの本菌が分離された.強毒株は7つの牧場から分離され,分離率は2%(508株中10株)であった.10株の強毒株のうち,4株は日本の木曽馬から分離された90-kb II型の病原性プラスミドを保有し,残りの6株はこれまでに報告のある10種類の病原性プラスミド(85-kb I, II, III, IV型; 87-kb I, II型; 90-kb I, II, III, IV型)DNAのEcoRIとEcdT22Iの制限酵素切断像の何れにもー致しない新しいプラスミド型を示した.90-kb II型のEcoRI像との類似性から,この新しいプラスミド型を90-kb V型と呼称した.これは,韓国・済州島における病原性プラスミドの初めて報告である.同じ病原性プラスミド型が韓国と日本の強毒株に認められたことは,韓国と日本の在来馬の由来,起源とその伝播を考える上で興味深い.

<i>Rhodococcus equi</i> was isolated from fecal and soil samples from four native Jeju horse farms and six Thoroughbred farms in Jeju, Korea. The isolates were examined for the presence of virulence-associated 15-17-kDa antigens (VapA) by colony blotting, using the monoclonal antibody 10G5, and for the gene encoding VapA by PCR. <i>R. equi</i> was isolated from all 36 soil samples collected from the 10 farms with between 5.0 × 10<sup>2</sup> and 7.5 × 10<sup>4</sup> colony-forming units (cfu) per gram of soil, and from 37 of 40 fecal samples with between 5.0 × 10<sup>1</sup> and 1.1 × 10 <sup>5</sup> cfu per gram of feces. Virulent <i>R. equi</i> was isolated from seven farms and appeared in 2.0% of isolates (10 of 508). Of the 10 virulent isolates, four contained a 90-kb type II plasmid, which has been found in isolates from the Kiso native horses of Japan, and the other six contained a new variant, which did not display the <i>Eco</i>RI and <i>Eco</i>T22I digestion patterns of the 10 representative plasmids already reported (85-kb types I, II, III, and IV; 87-kb types I and II; 90-kb types I, II, III, and IV). We designated the new variant as the "90-kb type V" plasmid, because its <i>Eco</i>RI digestion pattern is similar to that of the 90-kb type II plasmid. This is the first report of the prevalence of virulent <i>R. equi</i> in Jeju, Korea. The same virulence plasmid type is found in both Korean and Japanese isolates, providing insight into the origin, ancestry, and dispersal of native horses in Korea and Japan.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 65(12), 1313-1317, 2003-12-25

    Japanese Society of Veterinary Science

References:  31

Cited by:  6

Codes

  • NII Article ID (NAID)
    110003886153
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09167250
  • NDL Article ID
    6800692
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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