Enzymatic Characterization of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis
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- MIYOSHI Takeharu
- National Institute of Animal Health, National Agricultural Research Organization
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- TSUJI Naotoshi
- National Institute of Animal Health, National Agricultural Research Organization
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- ISLAM M. Khyrul
- National Institute of Animal Health, National Agricultural Research Organization
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- KAMIO Tsugihiko
- National Institute of Animal Health, National Agricultural Research Organization
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- FUJISAKI Kozo
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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In the present study, we performed enzymatic characterization of Haemaphysalis longicornis serine proteinase (HlSP) with a view to shed light on the mechanisms of blood digestion in the hard ticks. Escherichia coli-expressed recombinant HlSP (rHlSP) was shown to potently hydrolyze the synthetic substrates Bz-(DL)-Arg-pNA, Z-Ala-Ala-Leu-pNA and Suc-Ala-Ala-Ala-pNA and yielded an activity of 31.5, 88.2 and 18.3 μmol/min/mg protein, respectively at an optimum temperature of 25°C. However, the enzyme showed little activity to hydrolyze the substratese Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA and Pyr-Phe-Leu-pNA. The optimum pH for the enzyme was shown to be 4.0 to 5.0. Several inhibitors such as antipain, leupeptin and phenylmethylsulfonyl fluoride (PMSF), specific for serine proteinase were shown to inhibit enzyme activity by 20-82%, while E-64 (specific for cysteine proteinases) and pepstatinA (specific for aspartic proteinases) had shown only little inhibitory effects on it. This is the first report on enzymatic characterization of a functional serine proteinase from the hard ticks.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (10), 1195-1198, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681400786304
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- NII論文ID
- 110003886338
- 10013725402
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 7130050
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- PubMed
- 15528848
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可