Effect of Modified Oligopeptides from the Beetle Allomyrina dichotoma on Escherichia coli Infection in Mice
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- YAMADA Manabu
- National Institute of Animal Health
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- NAKAMURA Kikuyasu
- National Institute of Animal Health
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- SAIDO-SAKANAKA Hisako
- National Institute of Agrobiological Sciences
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- ASAOKA Ai
- National Institute of Agrobiological Sciences
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- YAMAKAWA Minoru
- National Institute of Agrobiological Sciences University of Tsukuba
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- SAMESHIMA Toshiya
- National Institute of Animal Health
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- MOTOBU Maki
- National Institute of Animal Health
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- HIROTA Yoshikazu
- National Institute of Animal Health
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抄録
The novel peptides based on a putative active site of defensin, an anti-bacterial peptide from the beetle Allomyrina dichotoma, were synthesized. These synthetic oligopeptides exhibited strong anti-bacterial activity in vitro, even against antibiotic-resistant pathogenic bacteria. Then, anti-bacterial activity of two newly synthesized peptides, RLYLRIGRR-NH2 (peptide A) and RLRLRIGRR-NH2 (peptide B) was also examined by macroscopic and histopathologic assessment during the course of infection in mice inoculated with antibiotic-resistant pathogenic Escherichia coli (E. coli) in vivo. Peptide B decreased the mortality of mice inoculated with antibiotic-resistant pathogenic E. coli. The results of macroscopic and histopathologic examinations revealed that peptide B could protect the mice from infection. In contrast, peptide A failed to protect mice from infection with antibiotic-resistant pathogenic E. coli. Also, modified peptides A and B produced no toxicity or side effects in mice. These results suggest that peptide B is useful for developing novel antibiotics against antibiotic-resistant pathogenic bacteria.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (2), 137-142, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426673920
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- NII論文ID
- 110003886369
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6863657
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- PubMed
- 15031540
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可