Molecular Cloning, Chromosomal Location, and Biological Activity of Porcine Interleukin-21
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- MUNETA Yoshihiro
- National Institute of Animal Health
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- KIKUMA Reiko
- National Institute of Animal Health
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- UENISHI Hirohide
- National Institute of Agrobiological Sciences
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- HOSHINO Tomoaki
- Kurume University School of Medicine
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- YOSHIHARA Kazuhiro
- National Institute of Animal Health
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- TANAKA Maiko
- STAFF-Institute
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- HAMASHIMA Noriyuki
- National Institute of Agrobiological Sciences
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- MORI Yasuyuki
- National Institute of Animal Health
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抄録
A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 μg/ml concanavalin A (ConA), 10 μg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 μg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identity to the bovine, human, and murine IL-21, respectively. The porcine IL-21 gene was mapped to porcine chromosome 8 (8q22→q23) by means of fluorescence in situ hybridization and radiation hybrid mapping, where the porcine IL-2 gene had been mapped nearby. The recombinant porcine mature IL-21 expressed by E. coli induced dose-dependent proliferation and IFN-γ production from a human NK cell line, NK0. The porcine IL-21 identified in this study will be helpful for the enhancement of innate immune responses of pigs.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (3), 269-275, 2004
公益社団法人 日本獣医学会
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詳細情報
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- CRID
- 1390282681403910656
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- NII論文ID
- 110003886393
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6901827
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- PubMed
- 15107555
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可