Expression of LacZ Gene in Canine Muscle by Intramuscular Inoculation of a Plasmid DNA

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  • MAEDA Sadatoshi
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • OHMORI Keitaro
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KURATA Keigo
    Department of Immunology, National Institute of Infectious Diseases
  • SAKAGUCHI Masahiro
    Department of Immunology, National Institute of Infectious Diseases
  • MASUDA Kenichi
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • OHNO Koichi
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • TSUJIMOTO Hajime
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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DNA immunization induces systemic humoral and cellular immune responses to the antigen encoded by cDNA in a plasmid DNA. In the present study, a plasmid DNA encoding cDNA of β-galactosidase (β-gal), pCAGGS-lacZ, was inoculated intramuscularly to a healthy dog in order to evaluate location and duration of the gene expression. On day 7, the plasmid DNA was found by PCR in the muscle where the plasmid was injected. Furthermore, β-gal expression was detected in the same muscle sample by β-gal staining. However, the plasmid DNA was not detected in any samples collected on days 14, 21 and 28. The present results suggest that duration of the gene expression of β-gal by the plasmid DNA is limited in the muscle in dogs and an efficacy for a gene expression should be evaluated depending on the gene inserted in the plasmid DNA for immunotherapy.<br>

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