Enzyme-Linked Immunosorbent Assay to Detect Lawsonia intracellularis in Rabbits with Proliferative Enteropathy

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  • WATARAI Masahisa
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • YAMATO Yosuke
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • HORIUCHI Noriyuki
    Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine
  • KIM Suk
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • OMATA Yoshitaka
    Department of Basic Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • SHIRAHATA Toshikazu
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
  • FURUOKA Hidefumi
    Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine

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Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.<br>

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