ザンビアLower Zambezi国立公園とLochinvar国立公園における野生動物の糞便とその生息環境土壌からのRhodococcus equiの分離 Isolation of Rhodococcus equi from the feces of indigenous animals and soil from the Lower Zambezi National Park and Lochinvar National Park, Zambia

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Author(s)

    • 高井 伸二 TAKAI Shinji
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 安田 準 [他] YASUDA Jun
    • 岩手大学農学部附属動物病院 Veterinary Teaching Hospital, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
    • 佐々木 由佳子 SASAKI Yukako
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 堤 尚子 TSUTSUMI Hisako
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 宮川 恵美子 MIYAGAWA Emiko
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 和田 かや WADA Kaya
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 角田 勤 KAKUDA Tsutomu
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 椿 志郎 TSUBAKI Shiro
    • 北里大学獣医畜産学部獣医衛生学教室 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • 杉本 千尋 SUGIMOTO Chihiro
    • 帯広畜産大学原虫病研究センター National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

Abstract

Rhodococcus equiは子馬にとって重要な病原細菌であるが,アフリカの野生動物における分布はよく分かっていない.ザンビアの二つの国立公園の野生動物の糞便92検体とその生息環境土壌43検体からR. equiを分離した. 533分離株における毒力関連15-17 kDa 抗原(vaPA)および20 kDa 抗原(vapB)とその遺伝子の存在を,モノクローナル抗体を用いたコロニーブロット法とPCR法で検索した.糞便検体の75%,土壌検体の74%から1g当たり10^2〜10^4cfuのR. equiが分離された.分離株のおよそ20%が様々の大きさの潜在プラスミドを保有していた.野生動物の糞便とその生息環境上壌から強毒株は検出できなかったが,無毒株が広く分布していることが明らかとなった.

<i>Rhodococcus equi</i> is an important pathogen in foals; however, its incidence in African indigenous animals is poorly understood. Fecal samples (92 from nine indigenous species) and 43 soil samples were collected from two Zambian National Parks. The presence of <i>R. equi</i> was investigated and 533 isolates were tested for the presence of 15- to 17-kDa antigens (VapA) and a 20-kDa antigen (VapB) by immunoblotting and PCR. <i>R. equi</i> was isolated (10<sup>2</sup>-10<sup>4</sup> colony forming units/g) from 75% of fecal and 74% of soil samples. Neither antigen was detected; however, about 20% of the isolates contained cryptic plasmids of various sizes. There was no evidence of virulent <i>R. equi</i>, but the avirulent form was widespread in the animals and the soil.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 66(6), 743-746, 2004-06-25

    Japanese Society of Veterinary Science

References:  24

Cited by:  1

Codes

  • NII Article ID (NAID)
    110003886480
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09167250
  • NDL Article ID
    7012955
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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