Molecular Characterization of Theileria orientalis Piroplasm Protein Encoded by an Open Reading Frame (To ORF2) in a Genomic Fragment

• KIM Jung-Yeon

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• YOKOYAMA Naoaki

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• KUMAR Sanjay

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• INOUE Noboru

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• FUJISAKI Kozo

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• SUGIMOTO Chihiro

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

抄録

In the present study, a novel antigenic protein expressed in the piroplasm stage of Theileria orientalis was characterized. A 4,707 bp genomic fragment amplified by PCR contained two open reading frames (ORFs). The deduced amino acid sequence of the first ORF showed significantly high similarlity to the ubiquitin carboxy terminal hydrolases/proteases while the second ORF (To ORF2) showed homology to several surface antigens of plasmodia. To ORF2 was expressed to determine whether the protein product is expressed by the parasite. In western blot analysis, bovine antiserum from a T. orientalis-infected calf recognized the recombinant protein containing a C-terminal part of the ORF expressed by baculovirus system. Western blot analysis with the anti-To ORF2 mouse serum recognized a 48 kDa protein in T. orientalis piroplasm lysates. Indirect immunofluorescence antibody test by confocal scanning laser microscopic analysis showed that antisera against the recombinant protein recognized T. orientalis piroplasm in the infected erythrocyte. The results from this study indicate that To ORF2 protein is expressed at the piroplasm stage and is immunogenic. This novel antigenic To ORF2 protein could be exploited for vaccine development against bovine piroplasmosis.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 66 (8), 957-963, 2004

  公益社団法人 日本獣医学会

キーワード

• Apicomplexa
• parasite vaccine
• piroplasm
• <i>Theileria orientalis</i>

詳細情報

• CRID: 1390001206425516416
• NII論文ID: 110003886491
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.66.957
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 7076124
• PubMed: 15353847
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7076124
• Web Site: http://www.jstage.jst.go.jp/article/jvms/66/8/66_8_957/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004280654
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可