Partial Cloning of Prohibitin cDNA from Canine, Feline, Bovine, Equine, and Rabbit Liver mRNA by RT-PCR.

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  • MATSUYAMA Satoshi
    Laboratory of Radiology, Department of veterinary Science, College of Agriculture, Osaka Prefecture University
  • KUBO Kihei
    Laboratory of Radiology, Department of veterinary Science, College of Agriculture, Osaka Prefecture University
  • OHASHI Fumihito
    Laboratory of Veterinary Surgery, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
  • TAKAMORI Yasuhiko
    Laboratory of Radiology, Department of veterinary Science, College of Agriculture, Osaka Prefecture University

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タイトル別名
  • Partial cloning of prohibitin c DNA fro

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Prohibitin is the protein which has an inhibitory function in cell growth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cDNAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT-PCR, and their homology analysis. The sequences of these RT-PCR products were compared with each other as well as those reported for human and rat. The homology in this region of prohibitin cDNA was approximately 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR products are a part of prohibitin cDNA of each animal.

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