Application of Maximal Removal Rate of Indocyanine Green to the Determination of Hepatic Functional Mass in Conscious Rats.

• INAGE Fujio

  Tokyo Research and Development Center, Daiichi Pharmaceutical Co., Ltd.

• FURUHAMA Kazuhisa

  Tokyo Research and Development Center, Daiichi Pharmaceutical Co., Ltd.

書誌事項

タイトル別名

• Application of maximal removal rate of

抄録

We established a versatile method for the measurement of indocyanine green maximal removal rate (ICG Rmax) to detect hepatic functional mass in conscious rats using a repeated blood sampling procedure. On investigation of the optimal technical conditions, the appropriate intravenous administered doses of ICG were 2.5, 5, 10 or 20 mg/kg, and the best blood collection times for calculating plasma half-life at these doses were immediately before, and 4, 7 and 10 min after ICG injection. The interval among the respective ICG injections was more than 4 hr. In hepatectomized rats, the ICG Rmax value was reduced to about 50% and 20% of sham-operated rats in mean 2/3 and 4/5 liver resections, respectively, suggesting that it would almost extrapolate to hepatic surviving reserves under these experimental conditions. In rats treated subcutaneously with carbon tetrachloride (CCl₄, 0.1 and 0.25 ml/kg) thrice weekly during a 17-week period (120 days), a decrease in ICG Rmax value did not correlate with increases in serum alanine transaminase (ALT), alkaline phosphatase (ALP) and total bilirubin values throughout the experimental periods. However, the reduced ICG Rmax well correlated with decreases in serum albumin and cholinesterase (CHE) values from day 50. Histological examinations in the liver revealed that nodules of hepatocytes were separated by thick fibrous bands, defining the typical aspect of cirrhosis on day 30 to 90. These results suggest that the measurement of ICG Rmax is a valuable tool for the estimation of hepatic functional integrity in rats.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 59 (5), 335-340, 1997

  公益社団法人 日本獣医学会

キーワード

• CCl₄
• hepatectomy
• hepatic functional mass
• ICG Rmax
• rat

詳細情報

• CRID: 1390001206427124992
• NII論文ID: 110003915154
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.59.335
• COI: 1:STN:280:ByiA3srmvVM%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 4217689
• PubMed: 9192353
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4217689
• Web Site: http://www.jstage.jst.go.jp/article/jvms/59/5/59_5_335/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997192701
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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