Improved in vitro cultivation of <italic>Babesia</italic> caballi

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  • AVARZED Abgaandorjiin
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • IGARASHI Ikuo
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • KANEMARU Takumi
    The Epizootic Research Station, Equine Research Institute, The Japan Association
  • HIRUMI Kazuko
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • OMATA Yoshitaka
    Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine
  • SAITO Atsushi
    Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine
  • OYAMADA Takashi
    Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University
  • NAGASAWA Hideyuki
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • TOYODA Yutaka
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • SUZUKI Naoyoshi
    Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University

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タイトル別名
  • Improved in vitro Cultivation of Babesia caballi.
  • Improved in vitro cultivation of Babesi

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抄録

Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O2, 5% CO2 and 93% N2). All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.

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