オウム病クラミジアの増殖と各種抗原物質の出現時期に関する研究〔英文〕  [in Japanese] Kinetic Studies on the Appearance of Antigens of Chlamydia psittaci during Its Developmental Cycle  [in Japanese]

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Abstract

Chlamydis psittaci(C. psittaci)のIzawa-1株とP-1041株をそれぞれVero E 6細胞に接種し, 単クローン性抗体を用いた間接蛍光抗体法により経時的に感染細胞におけるクラミジア抗原の産生を検討した. 属特異的なリポ多糖類抗原は全増殖過程を通じて検出された. 一方, 蛋白抗原において, 各種単クローン性抗体で検出される抗原決定基は感染後一定の時期を経て検出されるものから全増殖過程を通じて検出されるものまで多様であった. また両株に交差反応を示す各種単クローン性抗体を用いて, 共通抗原の出現時期を検討したところ, その出現時期は株によって異なり, ホモ株でヘテロ株より早いものからヘテロ株でホモ株より早いものまで変化がみられた. 次に電子顕微鏡により細胞質封入体内に含まれるクラミジア粒子の経時的な形態変化を観察し, 単クローン性抗体による特異抗原の出現との関連を検討した結果, C.psittaciの蛋白抗原上の抗原決定基は基本小体と網様体に共通なもの, および基本小体に特異的なものの2群に分類された.

The kinetics of the antigen production of Chlamydia psittaci strains Izawa-1 and Pigeon-1041 (p-1041) was examined every 6 hr after infection up to 48 hr, by the indirect immunofluorescent antibody technique using monoclonal antibodies (MAbs). All three genus-specific antigenic determinants on lipopolysaccharide (LPS) appeared during the whole growth cycle. Antigenic determinants on proteins were, on the other hand, detected at various time periods from the early to the late stages of infection. However, a cross-reactive antigenic determinant on protein recognized by a MAb 3E9 was also detected during the whole growth cycle, similar to that on LPS. The time of appearance of common antigenic determinants on proteins of Izawa-1 and P-1041 was examined using cross-reactive MAbs, and it varied depending on heterologous and homologous MAbs. From the relationship between the detection of antigenic determinants and the morphological changes of chlamydial particles revealed by electron microscopy during the growth cycle, the antigenic determinants on proteins of Chlamydia psittaci were divided into two groups; one was specific to the elementary body and the other was coexisting in both the elementary body and the reticulate body.

Journal

  • The Journal of veterinary medical science

    The Journal of veterinary medical science 53(4), p691-697, 1991-08

    Japanese Society of Veterinary Science

Codes

  • NII Article ID (NAID)
    110003915418
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    JPN
  • ISSN
    09167250
  • NDL Article ID
    3748536
  • NDL Source Classification
    RB72(畜産--獣医学)
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  NII-ELS 
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