飼育下および野生エゾヒグマUrsus arctos yesoensisにおけるP-EIAキットによる血清中プロジェステロン値の測定

DOI Web Site Web Site PubMed 被引用文献7件

書誌事項

タイトル別名
  • Serum Progesterone Concentrations using P-EIA Kit in Captive and Free-Ranging Hokkaido Brown Bears, Ursus arctos yesoensis.
  • 飼育下および野生エゾヒグマUrsus arctos yesoensisにおけるP-EIAキットによる血清中プロジェステロン値の測定〔英文〕
  • シイクカ オヨビ ヤセイ エゾヒグマ Ursus arctos yesoens

この論文をさがす

抄録

Serum progesterone (P) concentrations using P-EIA kit (Ovucheck, Cambridge Life Science Co., Ltd.) were examined in 8 captive and 7 free-ranging female Hokkaido brown bears (Ursus arctos yesoensis). The intra- and inter-assay coefficients of variation were 8.9%, 12.6% and 16.6%, 22.7%, respectively, bdsed on 2 serum samples. There was a significant correlation between EIA and radioimmunoassay results based on 64 serum samples (r=0.725; p<0.01). Serum P concentrations were examined in 5 pregnant, 2 solitary non-pregnant bears and a lactating non-pregnant bear in captivity. Annual changes of P levels in pregnant bears were observed as a small elevation during the mating season (May-June), a re-elevation in September-October and a sharp elevation in November-December. The sharp elevation was suspected to reflect changes when implantation occurred. Annual changes of P levels in solitary non-pregnant bears were similar to those in pregnant bears. An annual change of P levels in a lactating non-pregnant bear maintained levels under 5 ng/ml. Two of 7 free-ranging bears exhibited P levels over 1 ng/ml and the birth of cubs was confirmed in the following year in 1 of the 2 bears. P concentrations of other free-ranging bears exhibited less than 1 ng/ml, and these bears were considered to be non-pregnant. It was concluded that P-EIA kit was available for measuring P concentrations in Hokkaido brown bears.

収録刊行物

被引用文献 (7)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ