書誌事項
- タイトル別名
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- Two-Step Polymerase Chain Reaction for Diagnosis of Scrub Typhus and Identification of Antigenic Variants of Rickettsia tsutsugamushi.
- 2段階PCR法によるRickettsia tsutsugamushiの検出および血清型の同定〔英文〕
- 2ダンカイ PCRホウ ニ ヨル Rickettsia tsutsugamus
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抄録
Two-step polymerase chain reaction (nested PCR) method was examined for the diagnosis of scrub typhus. Primers were derived from the type-specific antigen (TSA) gene DNA sequences of Rickettsia tsutsugamushi, Gilliam strain. These primers served to produce rickettsia-specific products in the amplification of template DNA prepared from all serovariants, Gilliam, Karp, Kato, Kawasaki, Kuroki and Shimokoshi strains, and the fragments of product after digestion with several kinds of restriction endonuclease showed the respective patterns to strain in acrylamide or agarose gel electrophoresis. The rickettsia-specific DNAs were also derived, by this nested PCR, by amplifying DNA from patients' bloods and mites from endemic areas, and the serotype of rickettsiae infected to these hosts could be identified from fragment patterns of the amplified products observed after endonuclease treatment. These results indicate that this PCR is sensitive and specific method not only for detection of rickettsial DNA in patient specimens and in mites, but also for the typing of rickettsiae infected to these hosts.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 55 (5), 749-755, 1993
公益社団法人 日本獣医学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390001206426452352
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- NII論文ID
- 110003915908
- 130003571758
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK2cXitV2ls78%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 3855457
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- PubMed
- 8286526
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可