輓用種牡馬における精液性状, 繁殖成績, 及び精液の保存性の関係

• トーレス ボジノ フェルナンド

• 佐藤 邦忠

• 岡 明男

• 菅野 幸夫

• 保地 真一

• 小栗 紀彦

• ブラウン ヨアヒム

書誌事項

タイトル別名

• Relationship among Seminal Characteristics, Fertility and Suitability for Semen Preservation in Draft Stallions.
• 輓用種牝馬における精液性状,繁殖成績,及び精液の保存性の関係〔英文〕
• バンヨウシュ ヒンバ ニ オケル セイエキ セイジョウ ハンショク セイセキ

抄録

Seminal characteristics, fertility and the response to semen preservation (liquid storage and cryopreservation) were evaluated in 4 Draft stallions (Percheron 2, Breton 2). Seminal characteristics (gel-free volume, sperm concentration, sperm morphology, percentage of motile spermatozoa) were assessed in 5 ejaculates from each of the 4 stallions. The fertility of the stallions was calculated retrospectively as the accumulated pregnancy rate over 3 breeding seasons. Five ejaculates from each of the stallions were subjected to liquid storage at 5°C. The percentage of motile spermatozoa (PMS) was assessed at 0, 24, 48 and 72 hr. Cryopreservation was done with a glucose-EDTA-lactose egg yolk extender and a skim milk-based extender containing clarified egg yolk (4 replicates per stallion). PMS and the percentage of spermatozoa with an intact plasma membrane (IPM) were evaluated before and after freezing. Although semen charatcteristics and fertility were within a physiological range, significant differences among stallions were found for gel-free volume, percentage of morphologically normal spermatozoa, PMS, and fertility but there was no clear relationship between these parameters. The response to liquid storage of diluted semen samples differed among stallions and best results were obtained in a stallion with the highest initial motility. The freezability of semen from the 4 stallions was poor with both of the freezing protocols (PMS range 13.8%-26.3% ; IPM range 19.5%-38.0%). Results of liquid storage and cryopreservation were not correlated in individual stallions and were not related to their fertility. Since predictability for the response to semen preservation is low, the suitability of individual stallions for semen preservation has to be examined carefully. To improve the freezability of semen from Draft stallions, currently employed standard Protocols for cryopreservation need to be modified.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 57 (2), 225-229, 1995

  公益社団法人 日本獣医学会

キーワード

• cooling
• cryopreservation
• Draft stallion
• semen
• stallion spermatozoa

詳細情報

• CRID: 1390282681400915200
• NII論文ID: 130003572091
• NII論文ID: 110003916246
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.57.225
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 3618315
• PubMed: 7492637
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3618315
• Web Site: http://www.jstage.jst.go.jp/article/jvms1991/57/2/57_2_225/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996134727
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可