書誌事項
- タイトル別名
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- Preparation of Virus-Infection-Associated (VIA) Antigen of Foot-and-Mouth Disease (FMD) Virus from Inactivated Vaccine.
- Preparation of virus infection associat
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抄録
Virus-infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus was prepared from an inactivated FMD vaccine. The VIA antigen coupled with an adjuvant of aluminium hydroxide gel supplemented vaccine was efficiently eluted by suspending and stirring in high concentration of phosphate buffer solution (0.3M, pH 7.6). The final elute purified by DEAE-Sephadex A50 from the vaccine was concentrated in 1/500-1/1, 000 of the original volume. VIA antigens prepared from two kinds of vaccine (Type O and Asia-1) were antigenicaly identical to one prepared from a cell culture infected with live virus. Two cattle were infected with FMD virus (Type O) and sera were obtained from each cattle every week. The VIA antigens from the inactivated vaccine were compared with the ones from the cell culture infected with live virus in agar gel diffusion tests using sera from cattle infected with live virus. The antibodies against the VIA antigen were detected between the second week and the 13th or 14th week after infection. The VIA antigen from an inactivated vaccine would be very useful in FMD free countries like Japan to avoid the risk of using live FMD viruses.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 58 (6), 599-601, 1996
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426695680
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- NII論文ID
- 130003572426
- 110003916566
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK28XkvFKjsrg%3D
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- ISSN
- 13477439
- 09167250
- http://id.crossref.org/issn/09167250
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- NDL書誌ID
- 3976791
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- PubMed
- 8811637
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可