マレック病ウイルス血清2型糖蛋白I遺伝子: 遺伝子解析およびバキュロウイルスベクターを用いた発現 Marek's Disease Virus Serotype 2 Glycoprotein I Gene: Nucleotide Sequence and Expression by a Recombinant Baculovirus

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Author(s)

    • 張 炯寛 JANG Hyung-kwan
    • 東京大学農学部獣医微生物学教室 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tolyo
    • 小野 満 ONO Mitsuru
    • 東京大学農学部獣医微生物学教室 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tolyo
    • 蔡 錦順 CAI Jin-shun
    • 東京大学農学部獣医微生物学教室 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tolyo
    • 見上 彪 MIKAMI Takeshi
    • 東京大学農学部獣医微生物学教室 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tolyo

Abstract

マレック病ウイルス(MDV)血清2型(MDV2)相同糖蛋白I(gI homolog)のORFは, 355アミノ酸残基をコードし得る1065塩基からなり, MDV血清1型(MDV1)と血清3型(七面鳥ヘルペスウイルス: HVT)とはアミノ酸配列において各々49%, 36%の相同性が認められた. また予測されるN-linkの糖鎖付加部位並びにシグナル配列や膜貫通部位が存在し, 膜糖蛋白の性状を有していた. 転写産物解析により, このORFの翻訳開始コードンの上流56-147 bpで転写される3.5 kb mRNAがMDV2 gI homologの特異転写産物として同定された. MDV2(HPRS24株)感染鶏由来の抗血清を用いた免疫沈降解析によりMDV2 gI homologを発現するリコンビナントバキュロウイルス(rAcMDV2gI)の蛋白発現を検討したところ, rAcMDV2gI感染Sf9細胞では45-43 kDaの特異バンドが検出された. またツニカマイシン処理により糖鎖付加阻止試験を行ったところ, MDV2gI homologの前駆体蛋白と見られる35 kDaのバンドが検出された. これらの発現蛋白はホモのみならずヘテロの血清型MDV(GA株, SB-1株, FC126株)感染鶏由来の抗血清によっても認識されたことから, 型間共通のエピトープの存在並びに3血清型によるMDV感染細胞でのgIの発現が示唆された.

In the Marek's disease virus (MDV) serotype 2 (MDV2) genome, a gene equivalent to the glycoprotein I (gI) of other alphaherpesviruses was identified and sequenced. The primary translation product comprises 355 amino acids with a M_r of 38.4 kDa. The predicted amino acid sequence possesses several characteristics typical of membrane glycoproteins, including a N-terminal hydrophobic signal sequence, C-terminal transmembrane and cytoplasmic domains, and extra-cellular region containing three potential N-linked glycosylation sites. Compared to other MDV serotypes, MDV2 gI showed 49% identity with MDV1 gI, and 36% identity with HVT gI at the amino acid level. In transcriptional analyses, a 3.5 kb mRNA which starts between 56 and 147 bps upstream of the potential translational initiation codon of gI was identified as the gI-specific transcript. By a recombinant baculovirus, this potential gI encoding region was expressed as two specific products 45 and 43 kDa. Both products were susceptible to tunicamycin treatment, indicating that they were glycoprotein. Further, the expressed gI reacted with all chicken-antisera raised to each of the three serotypes of MDV (strains GA, SB-1, and FC126), suggesting that gI is expressed by all three serotypes of MDV in infected cells and conserves common antigenic epitope(s) beyond serotypes.

Journal

  • The Journal of veterinary medical science

    The Journal of veterinary medical science 58(11), 1057-1066, 1996-11-25

    Japanese Society of Veterinary Science

References:  54

Cited by:  6

Codes

  • NII Article ID (NAID)
    110003916648
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09167250
  • NDL Article ID
    4088278
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  CJPref  NDL  NII-ELS 
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