標識ストレプトアビジンビオチン(LSAB)法を用いたラットカーバチルスの検出(短報)

• Oros Jorge

• 松下 悟

• Rodriguez Jose L.

• Rodriguez Francisco

• Fernandez Antonio

書誌事項

タイトル別名

• Demonstration of Rat CAR Bacillus Using a Labelled Streptavidin Biotin (LSAB) Method.
• Demonstration of rat CAR bacillus using

抄録

Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 58 (12), 1219-1221, 1996

  公益社団法人 日本獣医学会

キーワード

• CAR bacillus
• immunohistochemistry
• rat

詳細情報

• CRID: 1390001206426224640
• NII論文ID: 130003572319
• NII論文ID: 110003916677
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.58.12_1219
• COI: 1:STN:280:DyaK2s7kvF2isw%3D%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 4129906
• PubMed: 8996705
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4129906
• Web Site: http://www.jstage.jst.go.jp/article/jvms1991/58/12/58_12_1219/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997083336
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可