Eimeria tenella の培養感染漿尿膜細胞内における発育

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  • Development of Eimeria tenella in Cultured Cells from the Parasitized Chorioallantoic Membrane
  • Eimeria tenella ノ バイヨウ カンセンショウニョウ マク サイボウ ナイ ニ オケル ハツイク

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The chorioallantoic membranes (CAM) of developing chick embryos infected with artificially excysted sporozoites of Eimeria tenella, were dispersed with trypsin-versene. These cells were cultured in Leighton tubes and observed at different times after cultivation. In this experiment, a closed culture system was applied in place of the CO2 gas phase system [7]. Results obtained were as follows. Schizogony: Five types of mature schizonts were found in cultured cells derived from CAM 3 to 6 days after inoculation. The morphological characters of these schizonts and merozoites coincided with those described by Long [7], though type 1 schizonts in this experiment were rather small in size (Fig. 1). Gametogony: All the developmental stages, including both sexual forms and oocysts, were found in cultured cells derived from CAM 4 to 6 days after inoculation. No attempts were made, however, to subinfect susceptible chickens with newly produced oocysts, for no sufficient number of materials could be harvested in this experiment.

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