6価クロムの細胞毒性に対する銅化合物の拮抗作用

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  • Antagonistic Effect of Cupric Compounds on Chromium Toxicity in the Growth of HeLa Cells
  • 6カ クロム ノ サイボウ ドクセイ ニ タイスル ドウ カゴウブツ ノ キッ

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To study the effects of various metal compounds on the cytotoxicity induced by hexavalent chromium of potassium dichromate, HeLa cells were incubated in Eagle's minimum essential medium (MEM) with or without the chromate alone, or both chromate and any one of cupric sulfate, zinc sulfate, cadmium nitrate, lead acetate and sodium selenite. After a given period of incubation, the growth and the chromium amount of the cells were calculated. The results obtained are summarized as follows. 1) The growth of HeLa cells in MEM with 1.7μM chromate at 3 days of incubation was approximately 60% of that of the control in MEM without the chromate. The growth of HeLa cells in MEM containing the above-mentioned concentration of the chromate present with 1.6μM or 8μM cupric sulfate was approximately 80% in both concentrations of the cupric compound. Furthermore, 8.0μM cupric compound could remarkably restore the growth inhibited with 3.4μM chromate. Thus no remarkable restorative effect on growth was observed by 1.4 to 140μM zinc sulfate, 5.2 nM to 3.2μM cadmium nitrate, 0.21 to 26μM lead acetate, or 4.6 to 580 nM sodium selenite present with the chromate in MEM. 2) The chromium amount (as Cr) of HeLa cells in MEM with 1.7μM chromate at 9 hours of incubation was 0.16μg/106 cells. When 8.0μM cupric compound was present in MEM with 1.7μM chromate, the chromium amount decreased to 1.0μg/106 cells, indicating 40% reduction. Other metal compounds, such as 140μM zinc sulfate, 26μM cadmium nitrate, 5.3μM lead acetate, and 0.58μM sodium selenite, which existed with the chromate in MEM also showed a tendency to reduce the chromium amount. Nevertheless, the rate of reduction was considerably lower than that by the cupric compound. Values under 15% without dose-dependency were observed. 3) When 8.0μM cupric acetate, cupric nitrate, or cupric chloride existed with 3.4μM chromate in MEM, the chromium amount of HeLa cells at 9 hours of incubation was 50 to 60% of that in MEM containing no cupric compound. The results mentioned above suggested that cupric compounds might have inhibited the chromium uptake of HeLa cells from the surrounding medium, which was closely related with the restorative effect of cupric compound on the chromium-induced cytotoxicity.

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