ラット脾臓の微細血管系における陰性荷電の電顕組織化学的研究 Electron Microscopic Cytochemical Studies of Anionic Sites in the Rat Spleen

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Author(s)

    • 植田 弘美 UEDA Hiroim
    • 酪農学園大学獣医学部獣医解剖学教室 Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University
    • 竹花 一成 TAKEHANA Kazushige
    • 酪農学園大学獣医学部獣医解剖学教室 Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University
    • 岩佐 憲二 IWASA Kenji
    • 酪農学園大学獣医学部獣医解剖学教室 Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University
    • 藤森 修 FUJIMORI Osamu
    • 名古屋市立大学医学部解剖学第二講座 2nd Department of Anatomy, Nagoya City University Medical School

Abstract

ラット脾臓の血管内皮細胞および脾索の細網細胞自由面の形質膜糖衣に発現する陰性荷電の強さを灌流固定を施した後, 陰性荷電を検出するpolyethyleneimine(PEI)溶液で灌流し, 電顕組織化学的手法を用いて明らかにした.動脈系血管として脾柱動脈, 中心動脈, 動脈性毛細血管, 静脈系血管として脾洞, 脾髄静脈, 脾柱静脈, さらには脾索の細網細胞についても検索した.脾柱動脈, 中心動脈, 動脈性毛細血管の動脈系血管や脾臓静脈および脾柱静脈において, 内皮細胞の形質膜糖衣に明瞭なPEI陽性反応が観察された.特に脾柱動脈, 中心動脈, 脾柱静脈では陽性反応像は帯状を呈し, 170-250nmの厚さであった.他方, 脾洞のい内皮細胞や脾索の細網細胞の同部位は明らかに弱い反応性を示し, 他の血管よりもPEI反応像は非常に薄いものであった.これらのことから, 赤脾臓に血液を供給する動脈系血管と血液を排出する静脈系血管では形状膜糖衣に発現する陰性荷電は強く, 赤脾臓を構成する脾洞の内皮細胞と細網細胞では形状膜糖衣に含まれる陰性荷電が極めて弱いことが明らかになった.このことは脾臓の血液循環や血液貯蔵などの生理学的機能に大いに関与しているものと推察された.

The distribution patterns of the intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of endo-thelial cells (ECs) in blood vessels and reticular cells (RCs) in the splenic cord of the rat spleen were studied by an electron microscopic cytochemical method using polyethyleneimine (PEI) as a cationic probe. Spleens from adult male rats were perfusion-fixed with 0.5% glutaraldehyde - 4 % paraformaldehyde containing 0.05% cetylpyridinium chloride and then perfused with 0.5% PEI at pH 7.4. On the free surfaces (glycocalyx of the plasma membrane) of the ECs examined, distinct PEI-positive reactions were observed in blood vessels, such as trabecular arteries, central arteries, arterial capillaries, pulp veins and trabecular veins. These PEI-positive electron-dense substances in the trabecular arteries, central arteries, and trabecular veins took the shape of a band of 170-250 nm in thickness. On the other hand, the corresponding ultrastructure of the ECs lining the splenic sinuses and the RCs in the splenic cord showed exceedingly weak PEI reactions. The PEI-reactive deposits were significantly thinner than those in the above blood vessels. As the thickness of the electron-dense substances can be related to the density of the negative charge, these results suggest that there is a high intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of ECs in blood vessels where blood cells and plasma pass into the red pulp or are discharged from the red pulp. In contrast, the splenic sinuses and RCs, which are the main components of the red pulp, contain weakly negative-charged sites. This may contribute to the microcirculation of the splenic blood vessels and elucidate the possible physiological functions of the spleen, such as blood storage.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 63(3), 287-291, 2001-03-25

    Japanese Society of Veterinary Science

References:  28

Codes

  • NII Article ID (NAID)
    110003920597
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09167250
  • NDL Article ID
    5715777
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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