MDSを用いた馬主席卵胞のLHとPGF_<2α>感作試験(臨床繁殖学) Effects of LH and PGF_<2α> in Equine Dominant Follicles Observed by MDS (Theriogenology)
-
- 小島 ゆみこ アンナ Y.KOJIMA Ana
- 帯広畜産大学獣医学科家畜臨床繁殖学教室:岐阜大学大学院連合獣医学研究科 Obihiro University of Agriculture and Veterinary Medicine:The United Graduate School of Veterinary Sciences, Gifu University
-
- 小林 修一 KOBAYASHI Shuichi
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- トマス ジャビエル アコスタ アヤラ [他] Tomas J.ACOSTA
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 工藤 眞樹子 KUDO Makiko
- 熊本市食肉衛生検査所 Meat Hygiene Inspection Center of Kumamoto City
-
- 宮本 明夫 MIYAMOTO Akio
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 高木 光博 TAKAGI Mitsuhiro
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 宮澤 清志 MIYAZAWA Kiyoshi
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 佐藤 邦忠 SATO Kunitada
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
この論文にアクセスする
この論文をさがす
著者
-
- 小島 ゆみこ アンナ Y.KOJIMA Ana
- 帯広畜産大学獣医学科家畜臨床繁殖学教室:岐阜大学大学院連合獣医学研究科 Obihiro University of Agriculture and Veterinary Medicine:The United Graduate School of Veterinary Sciences, Gifu University
-
- 小林 修一 KOBAYASHI Shuichi
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- トマス ジャビエル アコスタ アヤラ [他] Tomas J.ACOSTA
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 工藤 眞樹子 KUDO Makiko
- 熊本市食肉衛生検査所 Meat Hygiene Inspection Center of Kumamoto City
-
- 宮本 明夫 MIYAMOTO Akio
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 高木 光博 TAKAGI Mitsuhiro
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 宮澤 清志 MIYAZAWA Kiyoshi
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
-
- 佐藤 邦忠 SATO Kunitada
- 帯広畜産大学獣医学科家畜臨床繁殖学教室 Obihiro University of Agriculture and Veterinary Medicine
抄録
Microdialysisシステム(MDS)は, 異なる細胞間の内分泌学的解析を分子レベルで行う方法である.今回, 馬の排卵前卵胞液中プロジェステロン(P_4), エストラジオール17β(E_2), アンドロステンジオン(A_4)とプロスラグランジンF_<2α>(PGF_<2α>)濃度を調べた.卵巣から卵胞を取り出し, ホルモン測定のため卵胞液を採取した.卵胞の大きさを大(30mm<), 中(29〜16mm)および小(15mm>)に区分けして, 各々の卵胞液中のホルモン濃度をEIA法により測定した.卵胞の大きさの違いで, P_4とPGF_<2α>濃度に有意差はなく, (A_4)濃度は大と中の卵胞間で差異を認めた.E_2濃度は, 中卵胞より大卵胞が高値であった.卵胞膜の小片にMDS操作を行い, LHを感作した時にP_4,A_4とPGF_<2α>分泌は増加した.PGF_<2α>を感作した時, P_4とA_4分泌の増加は認められたが, E_2の変動は小さかった.今回の結果から, 排卵前卵胞はLH感作により卵胞膜での黄体化とステロイドホルモン分泌を促進した.さらに, 発情周期のホルモン動態は, 他の動物種と大きく異なることが明らかとなったが, 主席卵胞が選択される機序は十分説明できなかった.
Microdialysis System (MDS) is a novel technique used for investigation of molecule secretion between different cell populations. Local hormonal secretion at follicular wall has been still unclear. This MDS study was used to determine progesterone (P_4), androstenedione (A_4), estradiol-17β (E_2) and Prostaglandin F_<2α> (PGF_<2α>) release in mare pre-ovulatory follicles. Follicles larger than 30 mm were isolated from the ovary and follicular fluid aspirated for hormone assay. Follicular fluid collected from small, middle and large follicles were analyzed by EIA. The concentrations of P_4 and PGF_<2α> were similar among the different sizes of follicles. The release of A_4 was observed in middle and large follicles. E_2 concentration was observed in middle follicles and was higher in large follicles compared with middle follicles. Follicular wall was cut and incubated for MDS and when LH was infused, there was an increase in P4 and A_4 release. PGF_<2α> release was considerably high after LH infusion compared to the control group. Infusion of PGF_<2α> increased P_4 and A_4 release but there was no change in E_2 release. This results suggest that in pre-ovulatory follicles, LH stimulates theca interna cells and also PGF_<2α> seemed to have a mediator role to induce steroid hormone production and luteinization of follicular cells. The nature of the mechanisms involved in selection of large follicles is still a perplexing research problem in reproduction.
収録刊行物
-
- The journal of veterinary medical science
-
The journal of veterinary medical science 64(2), 119-122, 2002-02-25
社団法人日本獣医学会
参考文献: 23件中 1-23件 を表示
-
1
- Control of onset of breeding season in the mare and its artificial regulation by progesterone treatment
-
ALEXANDER S. L.
J. Reprod. Fertil. 44(Suppi.), 307-318, 1991
被引用文献1件
-
2
- Administration of human chorionic gonadotrophins (HCG) to pregnant pony mares
-
ALLEN W. E.
The Veterinary Record 94, 505, 1974
被引用文献1件
-
3
- A comparison of the biochemical composition of equine follicular fluid and serum at four different stages of the follicular cycle
-
COLLINS A.
Equine Vet. J. 25(Suppl.), 12-16, 1997
被引用文献1件
-
4
- The normal estrous cycle
-
DAELS P. F.
Equine Reproduction., 121-132, 1993
被引用文献1件
-
5
- Determination of secretion rates of oestradiol, progesterone, oxytocin, and angiotensin II from tertiary follicles and freshly formed corpora lutea in freely moving sows
-
EINSPANIER A.
Endocrinology 129, 3403-3409, 1991
被引用文献1件
-
6
- <no title>
-
GINTHER O. J.
Reproductive Biology of the Mare: Basic and Applied Aspects. Cross Plains, 1992
被引用文献1件
-
7
- Morphological and biochemical correlates of equine ovarian follicles as a function of their state of viability or atresia
-
KENNEY R. M.
J. Reprod. Fertil. 27(Suppl.), 163-171, 1979
被引用文献1件
-
8
- Effects of basic fibroblast growth factor, transforming growth factor on the secretory function of the bovine corpus luteum in vitro
-
MIYAMOTO A.
J. Endocrinology 135, 103-114, 1992
被引用文献1件
-
9
- <no title>
-
PALMER E.
J. Reprod. Fertil. 54, 495-505, 1978
被引用文献1件
-
10
- Induction of ovulation in cyclic mares by administration of a synthetic prostaglandin, fenprostalene, during oestrus
-
SAVAGE N. C.
J. Reprod. Fertil. 35(Suppl.), 239-243, 1987
被引用文献1件
-
11
- Developmental changes in steroidogenesis by equine preovulatory follicles: effects of equine LH, FSH, and CG
-
SIROIS J.
Endocrinology 127, 2423-2430, 1990
被引用文献1件
-
12
- Steroidogenesis by equine preovulatory follicles: relative roles of theca interna and granulosa cells
-
SIROIS J.
Endocrinology 128, 1159-1166, 1991
被引用文献1件
-
13
- In vitro steroidogenesis by granulosa cells from equine pre-ovulatory follicles
-
TUCKER K. E.
J. Reprod. Fertil. 44(Suppl.), 45-55, 1991
被引用文献1件
-
14
- Local release of steroid hormones, prostaglandin E_2, and endothelin-1 from bovine mature follicles in vitro: Effects of luteinizing hormone, endothelin-1 and cytokines
-
ACOSTA TJ
Biol. Reprod. 59, 437-443, 1998
DOI 被引用文献11件
-
15
- Immunohistochemical localization of cytochrome P450 aromatase in equine gonads
-
ALMADHIDI J.
J. Histochem. Cytochem. 43, 571-577, 1995
被引用文献4件
-
16
- Steroid concentrations in follicular fluid aspirated repeatedly from transitional and cyclic mares
-
BOGH I. B.
Theriogenology 54, 877-888, 2000
DOI 被引用文献1件
-
17
- Changes in LH pulse frequency and amplitude in intact mares during the transition into the breeding season
-
FRITZGERALD B. P.
J. Reprod. Fertil. 79, 485-493, 1987
DOI 被引用文献1件
-
18
- Follicle deviation and intrafollicular estradiol concentration in mares
-
GASTAL E. L.
Biol. Reprod. 61, 31-39, 1999
DOI 被引用文献1件
-
19
- Role of luteinizing hormone in follicle deviation based on manipulating progesterone concentrations in mares
-
GASTAL E. L.
Biol. Reprod. 61, 1492-1498, 1999
DOI 被引用文献1件
-
20
- Induction of Estrus and Promotion of Fertility by Prostaglandin F_2a Administration in Mares
-
KANEKO Masayuki , MIYAKE Yoh-Ichi , KANEDA Yoshihiro , WATANABE Gen , TAYA Kazuyoshi
Journal of equine science 6(1), 7-14, 1995-03
J-STAGE 参考文献28件 被引用文献4件
-
21
- Effect of periovulatory prostaglandin F_<2α> on pregnancy rates and luteal function in the mare
-
TROEDSSON M. H. T.
Theriogenology 55, 1891-1899, 2001
DOI 被引用文献1件
-
22
- Changes in the concentrations of steroids and prostaglandin F in preovulatory follicles of the mare after administration of hCG
-
WATSON E. D.
J. Reprod. Fertil. 84, 557-561, 1988
DOI 被引用文献1件
-
23
- Local distributions of oviductal estradiol, progesterone, prostaglandins, oxytocin and endothelin-1 in the cyclic cow
-
WIJAYAGUNAWARDANE MP
Theriogenology 49, 607-618, 1998
DOI 被引用文献6件