Bacteriology: Characterization of a neutralizing monoclonal antibody against botulinum ADP-ribosyltransferase, C3 exoenzyme
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- KAMATA Yoichi
- Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
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- HOSHI Hidenobu
- Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
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- CHOKI Hayato
- Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
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- KOZAKI Shunji
- Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
書誌事項
- タイトル別名
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- Characterization of a Neutralizing Monoclonal Antibody against Botulinum ADP-Ribosyltransferase, C3 Exoenzyme.
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抄録
A monoclonal antibody, named C302, was prepared and characterized against botulinum ADP-ribosyltransferase C3 exoenzyme that inactivates RhoA GTP-binding protein, resulting in the neurite outgrowth of human neuroblastoma GOTO cells. C302 bound not to the smaller fragments derived from the protease-treated C3 exoenzyme but to the intact C3 exoenzyme. It seems that the C302 epitope may depend on the three-dimensional structure of C3 exoenzyme molecule. C302 depressed the enzymatic and biological actions of C3 exoenzyme. The dose-dependent depression pattern of C302 on the enzyme activity was similar to that to the biological one. C302 turned the neurite-bearing shape of the C3 exoenzyme-treated GOTO cells into the intact shape. By using of C302 mAb and C3 exoenzyme, the research concerning GTP-binding proteins would be improved.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 64 (9), 767-771, 2002
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681402854144
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- NII論文ID
- 110003920937
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38XosFSku7c%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6312061
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- PubMed
- 12399599
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可