Toxicology: cDNA cloning and expressions of cytochrome P450 1A in zebrafish embryos

DOI Web Site Web Site PubMed 被引用文献4件 参考文献27件
  • YAMAZAKI Koji
    Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University
  • TERAOKA Hiroki
    Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University
  • DONG Wu
    Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University
  • STEGEMAN John J.
    Woods Hole Oceanographic Institution
  • HIRAGA Takeo
    Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University

書誌事項

タイトル別名
  • cDNA Cloning and Expressions of Cytochrome P450 1A in Zebrafish Embryos.

この論文をさがす

抄録

Cytochrome P450 1A (CYP1A) is well known for being induced by aromatic hydrocarbons, including 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). We determined the complete cDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish (zfCYP1A), a useful model for environmental toxicology. zfCYP1A shows high percentage identity with CYP1As of mammals, domestic fowl and xenopus (51.9-60.4%), as well as the other fish species (63.8-89.2%). As revealed by in situ hybridization and immunohistochemistry, zfCYP1A was scarcely detected in control embryos but was markedly induced by TCDD especially in heart, vascular endothelial cells, intestinal epithelium, pronephros and outer integument in both prehatched and hatched embryos. These expression patterns are consistent with possible involvement of zfCYP1A in TCDD-induced toxicities.

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ