IgAモノクローナル抗体受動免疫による旋毛虫のマウス腸管上皮への定着阻害 Impeded Establishment of the Infective Stage of Trichinella in the Intestinal Mucosa of Mice by Passive Transfer of an IgA Monoclonal Antibody

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Abstract

IgAモノクローナル抗体HUSM-Tb1は旋毛虫(Trichlnella britovi)感染幼虫の体表に免疫沈降物を形成し,また感染5時間前に腹腔内注射することでT.britoviのマウス腸管感染を顕著に抑制することができることを以前に報告した.この抗体は,他種旋毛虫(Trichinella pseudospiralis)に対しても同様の効果をもつことが確認され,少なくともTrichinella属に共通した抗原に特異性をもつことが示唆された.本IgA抗体を受動免疫したマウスでは,旋毛虫感染幼虫を経口投与した1時間後に腸管上皮内に定着した幼虫はほとんどなく,一方,抗体移入を行わない対照群ではかなりの数の幼虫がすでに粘膜に定着していた.このことは,このIgAモノクローナル抗体によって感染幼虫の宿主腸管上皮への侵入が阻害されることを示唆している.紫外線照射旋毛虫幼虫を用いて経口免疫したマウスに同種チャレンジ感染を行うと,感染後4〜7日目に腸管からの排虫がみられた.IgAモノクローナル抗体HUSM-Tb1は紫外線照射旋毛虫により反復経口免疫したマウスの腸間膜リンパ節に由来するが,この免疫モデル自体ではIgA抗体が仲介する粘膜虫体排除機構は働いていないと考えられた.

Our previous study showed that the IgA monoclonal antibody (mAb) HUSM-Tb1 forms immunoprecipitates on the cuticular surface of infective larvae of <i>Trichinella britovi</i>, and that intraperitoneal injection of this mAb to mice 5 hr before challenge infection confers a high level of protection against intestinal <i>T. britovi</i>. The same treatment produced a similar effect in BALB/c mice inoculated orally with <i>Trichinella pseudospiralis</i> larvae, indicating that the effects may be seen upon most members of the genus <i>Trichinella</i>. Worms recovered from the intestinal mucosa at 1 hr after challenge infection with <i>T. pseudospiralis</i> was few in mice passively immunized with the mAb, whereas a substantial number of worms were recovered from the mucosa of control groups. These results suggest that the IgA mAb impedes establishment of infective <i>Trichinella</i> worms in the intestinal mucosa. <i>Trichinella</i> worms inoculated orally into BALB/c mice vaccinated with ultraviolet-irradiated muscle larvae 3 weeks earlier were expelled between days 4 and 7 after challenge infection. Although the mAb HUSM-Tb1 originated from the mesenteric lymph node cells of mice vaccinated repeatedly with such irradiated larvae, IgA-mediated expulsion does not seem to play an important role in this vaccination model.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 65(11), 1227-1231, 2003-11-25

    Japanese Society of Veterinary Science

References:  29

Codes

  • NII Article ID (NAID)
    110003920986
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09167250
  • NDL Article ID
    6926796
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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