Imaging Elastic Properties of Soft Materials Immersed in Water Using Force Modulation Mode in Atomic Force Microscopy.

  • Haga Hisashi
    Divisions of Physics Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Sasaki Shigeo
    Divisions of Physics Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Morimoto Mayumi
    Divisions of Physics Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Kawabata Kazushige
    Divisions of Physics Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Ito Etsuro
    Divisions of Biological Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Abe Kazuhiro
    Department of Anatomy, Hokkaido University School of Medicine, Kita–ku, Sapporo 060, Japan
  • Sambongi Takashi
    Divisions of Physics Sciences, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan

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タイトル別名
  • Imaging Elastic Properties of Soft Mate

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Using the force modulation mode in atomic force microscopy (AFM), we have succeeded in imaging elastic properties of agar gels immersed in water. The elastic images of agar have been captured simultaneously with the topographic images. Stiffer grains of agar whose size is about 200 nm can be clearly seen in the elastic image of 3.0% agar, while they are not so visible in the case of 1.5% agar. These grains probably correspond to aggregation of agar which cannot be observed in the topographic images. We also measured force-versus-distance curves using AFM to confirm that the absolute values of elastic modulus (Young's modulus) of agar coincide with the bulk values measured using the conventional stress-strain method. The estimated values of the elastic moduli with the AFM were 40 and 90 kPa for 1.5% and 3.0% agar gels, respectively. These are in good agreement with the respective bulk values of 30 and 80 kPa obtained using the conventional method.

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