Electroejaculation and Semen Cryopreservation of Free-Ranging Japanese Black Bears (Ursus thibetanus japonicus)
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- OKANO Tsukasa
- The United Graduate School of Veterinary Science, Gifu University
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- MURASE Tetsuma
- The United Graduate School of Veterinary Science, Gifu University Laboratory of Theriogenology, Faculty of Applied Biological Sciences, Gifu University
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- TSUBOTA Toshio
- The United Graduate School of Veterinary Science, Gifu University Laboratory of Zoo and Wildlife Medicine, Faculty of Applied Biological Sciences, Gifu University
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抄録
The Japanese black bear (Ursus thibetanus japonicus) is endangered for extinction in some areas of Japan, and semen collection and cryopreservation are an important means to preserve genetic resources. The aim of this study was to characterize and cryopreserve semen of free-ranging Japanese black bears. Semen was collected by electroejaculation procedure from 4 free-ranging Japanese black bears at the capture point in the field. Ejaculates containing motile sperm were recovered from all of the animals and ejaculate volume, total sperm count, % motility (percentage of motile spermatozoa), % viability (percentage of spermatozoa that excluded eosin) and % abnormal morphology (range (mean)) were 0.65-2.20 (1.51) ml, 99-1082 (490) × 106, 5-100 (31), 42-97 (66) and 20-87 (53), respectively. Three of the 4 ejaculates were diluted with an egg yolk-TRIS-citrate-glucose extender and cryopreserved in liquid nitrogen. Motile spermatozoa were observed after freezing and thawing in all cases. This study showed that electroejaculation would be a useful method for collecting semen from free-ranging Japanese black bears and that at least motile spermatozoa would be obtained by freezing the thus collected electroejaculates.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (11), 1371-1376, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681400957696
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- NII論文ID
- 110003963617
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 7166002
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- PubMed
- 15585951
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可