犬の膜結合型内因性マトリクスメタロプロテイナーゼインヒビターRECKのクローニング

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献6件 参考文献35件
  • TAKAGI Satoshi
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University
  • KITAMURA Takanori
    Department of Pharmacology, Graduate School of Medicine, Kyoto University
  • HOSAKA Yoshinao
    Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University
  • OHSAKI Tomohiro
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University
  • BOSNAKOVSKI Darko
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University
  • KADOSAWA Tsuyoshi
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University
  • OKUMURA Masahiro
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University
  • FUJINAGA Toru
    Laboratory of Veterinary Surgery, Department of Clinical Science, Graduate School of Veterinary Medicine, Hokkaido University

書誌事項

タイトル別名
  • Molecular Cloning of Canine Membrane-Anchored Inhibitor of Matrix Metalloproteinase, RECK

この論文をさがす

抄録

The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation, migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.<br>

収録刊行物

被引用文献 (6)*注記

もっと見る

参考文献 (35)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ