加齢によるアキレス腱コラーゲンの可溶性変化:ラットのアキレス腱におけるコラーゲンの生化学的分析  [in Japanese] Age-Related Changes in the Solubilities of Achilles Tendon Collagen:Biochemical Studies on Collagen from Rat Achilles Tendon  [in Japanese]

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Abstract

今回我々はラットのアキレス腱における,コラーゲン線維の加齢変化を,その可溶性の変化を中心に検討することを目的とし実験を行った。実験動物は3週齢,9週齢および37週齢の雄のWistar系ラットを使用した。結果は,コラーゲン濃度については週齢の違いによる有意な変化は認めなかった。コラーゲン線維の可溶性の変化については,3-9週齢間の比較では,9週齢の塩可溶性コラーゲン線維のみ減少した。9-37週齢間の比較では,37週齢の酸可溶コラーゲンの減少と不溶性コラーゲンの増加を認めた。このことは,ラットのアキレス腱に関して性的に成熟期をむかえたころから既に,酸可溶コラーゲンの減少及び不溶性コラーゲンの増加の可能性を示唆している。組織の柔軟性に関しても,この頃から柔軟性が低下する方向ヘコラーゲン線維が変化しはじめると推察出来る。

The purpose of this study was to examine the effect of aging on collagen concentration and the change in solubility of Achilles tendon collagen. Wistar strain male rats, aged 3 (n=6), 9 (n=6) and 37 (n=6) weeks, were used. Hydroxyproline was determined for the estimation of the collagen content in neutral salt soluble, acid soluble and insoluble collagen. The collagen concentration in Achilles tendon was expressed as a per cent of wet weight. The collagen concentration did not change significantly between 3 and 9 weeks of age, 9 and 37 weeks of age. The percentage of the salt soluble collagen to the total collagen was decreased significantly between 3 and 9 weeks of age, and did not change significantly between 9 and 37 weeks of age. The percentage of the acid soluble collagen to the total collagen was decreased significantly between 9 and 37 weeks of age, and did not change significantly between 3 and 9 weeks of age. The percentage of the insoluble collagen to the total collagn was increased significantly between 9 and 37 weeks of age, and did not change significantly between 3 and 9 weeks of age. These results suggest that 1) The collagen concentration in Achilles tendon may not change significantly with weeks of age. 2) The solubility of Achilles tendon, expressed as a percentage of the salt soluble collagen, acid soluble collagen and insoluble collagen to the total collagen, may change significantly with weeks of age.

Journal

  • Physical Therapy Japan

    Physical Therapy Japan 23(5), 269-274, 1996

    Japanese Society of Physical Therapy

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