Expression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Crystalline Silica In Vivo

Author(s)

    • OYABU Takako
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • OGAMI Akira
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • YAMATO Hiroshi
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • KURODA Kaori
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • OBATA Yasuko
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • HIGASHI Toshiaki
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health
    • TANAKA Isamu
    • Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health

Abstract

It has been theorized that Clara cell secretion protein (CCSP) plays a critical role in regulating the acute inflammatory response in the lung. We hypothesized that CCSP is also related to lung injury induced by occupational dust. The present study was conducted to investigate the time course of the expression of CCSP in lungs exposed to crystalline silica in vivo. Male Wistar rats were administered 1mg or 2mg of silica suspended in saline by a single intratracheal instillation and were sacrificed at 3d, 1wk, 1 month, 3 months and 6 months of recovery time. The expression of CCSP was observed by RT-PCR and western blot analysis. Exposure to 2mg of silica decreased in levels of CCSP mRNA at 3d, 1wk, 1 month and 6 months following intratracheal instillation. The protein level of CCSP in silica-exposed rats was decreased at 3d, 7d and 1 month after a single instillation of 2mg. The decreases in CCSP at the acute phase in this experiment suggest that CCSP may regulate the acute injury of the lung exposed to silica.

Journal

  • Journal of Occupational Health

    Journal of Occupational Health 47(6), 504-509, 2005-11-01

    Japan Society for Occupational Health

References:  18

Codes

  • NII Article ID (NAID)
    110004020576
  • NII NACSIS-CAT ID (NCID)
    AA11510660
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13419145
  • NDL Article ID
    7731131
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z54-J76
  • Data Source
    CJP  NDL  NII-ELS 
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