Delayed Induction of Telomere Instability in Normal Human Fibroblast Cells by Ionizing Radiation

Author(s)

    • Ojima Mitsuaki OJIMA Mitsuaki
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • Hamano Hiroki HAMANO Hiroki
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • Suzuki Masatoshi [他] SUZUKI Masatoshi
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • SUZUKI Keiji
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • KODAMA Seiji
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • WATANABE Masami
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University

Abstract

We examined the delayed induction of telomere instability in hTERT-immortalized normal human fibroblast (BJ1-hTERT) cells exposed to X-rays. BJ1-hTERT cells were irradiated with 2 Gy of X-rays, and chromosome aberrations were analyzed 24 hours after irradiation and in the surviving cells 14 days after X-ray exposure. We found that the X-ray-surviving cells showed an increased frequency of chromatid gaps and breaks and chromosome fragments compared to the control cells. Furthermore, centromere- and telomere-FISH revealed that the frequency of telomere loss and duplication significantly increased in surviving cells compared to the control level. Because no induction of telomere abnormality was observed in cells 24 hours after irradiation, X-irradiation might not affect telomeres directly, but it specifically induces delayed telomere instability in normal human fibroblast cells.<br>

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 45(1), 105-110, 2004-03-15

    Journal of Radiation Research Editorial Committee

References:  21

Cited by:  3

Codes

  • NII Article ID (NAID)
    110004040999
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    04493060
  • NDL Article ID
    6905701
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE  NDL-Digital 
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