Induction of Micronuclei in CHO Cells by Bleomycin but not by X-irradiation is Decreased by Treatment with HMG-CoA Reductase Inhibitors

Author(s)

    • Kodama Seiji KODAMA Seiji
    • Division of Radiation Biology and Health Science, Research Center for Radiation and Radioisotopes, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • SASAKI Hitoshi
    • Department of Hospital Pharmacy, Nagasaki University School of Medicine
    • TANIYAMA Kotaro
    • Department of Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University
    • WATANABE Masami
    • Laboratory of Radiation Biology, Divison of Radiation Life Science, Research Reactor Insutitute, Kyoto University

Abstract

We investigated the effect of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, pravastatin and fluvastation, on the induction of micronuclei by ionizing radiation or bleomycin in Chinese hamster ovary cells in order to assess the radical-scavenging ability of these inhibitors. The results indicated that both pravastatin and fluvastatin had no effect on the induction of micronuclei by X-irradiation when they were applied for either pre-treatment or post-treatment. In contrast, both drugs effectively reduced the frequency of bleomycin-induced micronuclei when they were applied for simultaneous treatment or post-treatment, but not for pre-treatment. This indicates that the radical-scavenging ability of these two HMG-CoA reductase inhibitors differs according to the origins of the radicals - e.g., X-rays or bleomycin - even when the two drugs are compared at an equivalent cytotoxic dose. Our results suggest that both pravastatin and fluvastatin have the ability to scavenge certain types of radicals and to protect cells against oxidative stress.

We investigated the effect of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, pravastatin and fluvastatin, on the induction of micronuclei by ionizing radiation or bleomycin in Chinese hamster ovary cells in order to assess the radical-scavenging ability of these inhibitors. The results indicated that both pravastatin and fluvastatin had no effect on the induction of micronuclei by X-irradiation when they were applied for either pre-treatment or post-treatment. In contrast, both drugs effectively reduced the frequency of bleomycin-induced micronuclei when they were applied for simultaneous treatment or post-treatment, but not for pre-treatment. This indicates that the radical-scavenging ability of these two HMG-CoA reductase inhibitors differs according to the origins of the radicals - e.g., X-rays or bleomycin - even when the two drugs are compared at an equivalent cytotoxic dose. Our results suggest that both pravastatin and fluvastatin have the ability to scavenge certain types of radicals and to protect cells against oxidative stress.<br>

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 46(2), 191-195, 2005-06-30

    Journal of Radiation Research Editorial Committee

References:  17

Codes

  • NII Article ID (NAID)
    110004041115
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    04493060
  • NDL Article ID
    7345048
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • Data Source
    CJP  NDL  NII-ELS  IR  J-STAGE  NDL-Digital 
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