女性ホルモンエストロゲンとアドレナリンの血管形成に与える影響 : 培養血管内皮細胞を用いた検討

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  • ジョセイ ホルモンエストロゲン ト アドレナリン ノ ケッカン ケイセイ ニ アタエル エイキョウ バイヨウ ケッカン ナイヒ サイボウ オ モチイタ ケントウ

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During morphogenesis in human development, stem cells or precursor cells of vascular system actively divide, move and form vascular tree in embryo (vaculogenesis). However, vascular system retains plasticity even after adult stage of human. Endothelial cells of vasculature in ischemic tissue are activated by humoral factors and change their phenotype. They restart to build neo-vasculature in ischemic region as an adaptation phenomenon (angiogenesis). Female hormone and exercise are two of the important humoral factors that modulate angiogenesis. Therefore, exercise in women may cause different effects on vasculature compared with men. Eventually, the incidence and severity of ischemic diseases are different between male and female. In this study, we examined the effects of a typical female hormone, 17β-estradiol and a typical humoral factor of exercise, adrenaline on angiogenesis using cultured vascular endothelial cells. When bovine aovtic endothelial cells cultured on a plastic dish were partially scraped off, cells were activated and migrated toward free space from the edge. We quantitated the distance of cell migration under microscopy. 17β-estradiol (100pM) and adrenaline (10nM) increased migration of endothelial cells, which was inhibited by specific estrogen receptor antagonist, ICI 182, 780 and β-adrenergic receptor antagonist, propranolol. Co-administration of 17β-estradiol (100pM) and adrenaline (10nM) decreased cell motility compared to 17β-estradiol or adrenaline alone. Neither ICI 182, 780 nor propranolol abrogated the effect of co-administration. These results suggest that humoral milieus of sex difference and exercise exert their effects on angiogenesis in a cellular model via specific receptors, respectively. They also showed synergistic effect on cell migration. However, the mechanism of synergy was not receptor-mediated. Therefore, when applying exercise on women, it should be noted that the result may not be similar with that on men.

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