Antiviral Activity of the Marine Alga Symphyocladia latiuscula against Herpes Simplex Virus (HSV-1) in Vitro and Its Therapeutic Efficacy against HSV-1 Infection in Mice
-
- Park Hye-Jin
- Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
-
- Kurokawa Masahiko
- School of Medicine, Toyama Medical and Pharmaceutical University
-
- Shiraki Kimiyasu
- School of Medicine, Toyama Medical and Pharmaceutical University
-
- Nakamura Norio
- Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
-
- Choi Jae-Sue
- Faculty of Food Science and Biotechnology, Pukyong National University, Korea
-
- Hattori Masao
- Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
この論文をさがす
抄録
The antiviral activities of extracts from 5 species of marine algae collected at Haeundae (Pusan, Korea), were examined using plaque reduction assays. Although the activity of a methanol (MeOH) extract of Sargassum ringoldianum (Sargassaceae) was the most potent against several types of viruses, it was also cytotoxic. A MeOH extract of Symphyocladia latiuscula (Rhodomelaceae) and its fractions exhibited antiviral activities against acyclovir (ACV) and phosphonoacetic acid (PAA)-resistant (APr) herpes simplex type 1 (HSV-1), thymidine kinase (TK−) deficient HSV-1 and wild type HSV-1 in vitro without cytotoxicity. The major component, 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (TDB) of a CH2Cl2-soluble fraction was active against wild type HSV-1, as well as APr HSV-1 and TK− HSV-1 (IC50 values of 5.48, 4.81 and 23.3 μg/ml, respectively). The therapeutic effectiveness of the MeOH extract and TDB from S. latiuscula was further examined in BALB/c mice that were cutaneously infected with HSV-1 strain 7401H. Three daily oral administrations of the MeOH extract and TDB significantly delayed the appearance of score 2 skin lesions (local vesicles) and limited the development of further score 6 (mild zosteriform) lesions in infected mice without toxicity compared with controls. In addition, TDB suppressed virus yields in the brain and skin. Therefore TDB should be a promising anti HSV agent.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 28 (12), 2258-2262, 2005
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679601297024
-
- NII論文ID
- 110004049127
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 7720912
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可