Prediction of Cellular Radiosensitivity from DNA Damage Induced by .GAMMA.-Rays and Carbon Ion Irradiation in Canine Tumor Cells

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  • WADA Seiichi
    Research Group for Biotechnology Development, Japan Atomic Energy Research Institute-Takasaki
  • KHOA Tran Van
    Department of Biology and Medical Geneties, Center of Radiation Health
  • KOBAYASHI Yasuhiko
    Research Group for Biotechnology Development, Japan Atomic Energy Research Institute-Takasaki
  • FUNAYAMA Tomoo
    Research Group for Biotechnology Development, Japan Atomic Energy Research Institute-Takasaki
  • OGIHARA Kikumi
    Department of Environmental Pathology, Azabu University
  • UENO Shunji
    Laboratory of Veterinary Public Health, School of Veterinary Medicine and Animal Sciences, Kitasato University
  • ITO Nobuhiko
    Laboratory of Veterinary Radiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

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Other Title
  • 犬腫瘍細胞におけるγ線照射と炭素線照射によるDNA損傷からの放射線感受性の予測
  • Prediction of Cellular Radiosensitivity from DNA Damage Induced by γ-Rays and Carbon Ion Irradiation in Canine Tumor Cells
  • Prediction of Cellular Radiosensitivity from DNA Damage Induced by ガンマ Rays and Carbon Ion Irradiation in Canine Tumor Cells

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Abstract

Diseases of companion animals are shifting from infectious diseases to neoplasms (cancer), and since radiation therapy is one of the effective choices available for cancer treatment, the application of radiotherapy in veterinary medicine is likely to increase. However tumor tissues have different radiosensitivities, and therefore it is important to determine the intrinsic radiosensitivity of tumors in individual patients in advance of radiotherapy. We have studied the relationship between the surviving cell fraction measured by a clonogenic assay and DNA double strand breaks detected by a comet assay under neutral conditions in three canine tumor cell lines, after γ-ray and carbon ion irradiation. In all the cell lines, cell death assessed by the clonogenic assay was much higher following irradiation with carbon ions than with γ-rays. The initial and residual (4 hr) DNA damage due to γ-ray and carbon ion irradiation were higher in a radiosensitive cell line than in a radioresistant cell line. The surviving cell fraction at 2 Gy (SF2) showed a tendency for correlation with both the initial and residual DNA damage. In particular, the residual damage per Gy was significantly correlated with SF2, regardless of the type of radiation. This indicates that cellular radiosensitivity can be predicted by detection of radiation-induced residual DNA damage.<br>

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