吸血中のフタトゲチマダニ (Haemaphysalis longicornis) 唾液腺由来cDNAライブラリーによる発現遺伝子の網羅的解析 Random Sequencing of cDNA Library Derived from Partially-Fed Adult Female Haemaphysalis longicornis Salivary Gland

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Author(s)

    • 中島 千絵 NAKAJIMA Chie
    • 北海道大学大学院獣医学研究科感染症学教室 Laboratory of Infectious Disease, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
    • 今村 彩貴 [他] IMAMURA Saiki
    • 北海道大学大学院獣医学研究科感染症学教室 Laboratory of Infectious Disease, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
    • 今内 覚 KONNAI Satoru
    • 北海道大学大学院獣医学研究科感染症学教室 Laboratory of Infectious Disease, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
    • 大橋 和彦 OHASHI Kazuhiko
    • 北海道大学大学院獣医学研究科感染症学教室 Laboratory of Infectious Disease, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
    • 小沼 操 ONUMA Misao
    • 北海道大学大学院獣医学研究科感染症学教室 Laboratory of Infectious Disease, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

Abstract

吸血中のフタトゲチマダニ(Haemaphysalis longicornis)より唾液腺を採取してcDNAライブラリーを作成し, ランダムに選んだ発現タンパク遺伝子の配列をblastx (NCBI)等のプログラムを用いて解析した.得られた633配列はその相同性によって, 同一あるいはごく近縁の遺伝子由来と思われる213配列に集約された.全633配列のうち, blastxを用いた相同性検索により発現タンパクの機能予測がなされたものは全体の36%であり, 残りの64%は未知の遺伝子であった.機能が予測されたタンパクの大半は細胞の生存に必要なハウスキーピングタンパクであったが, 血液凝固阻害因子類似の蛋白を含めたプロテアーゼインヒビターやメタロプロテアーゼ, 他のダニで分離されている免疫抑制物質に類似したタンパク遺伝子等も同定された.これらのタンパクはダニの吸血に際し何らかの重要な役割を演じている可能性があり, 新たな抗ダニワクチン抗原の候補となりうると考えられる.

A cDNA library was constructed from salivary glands of partially-fed adult female <i>Haemaphysalis longicornis</i> (hard tick). Randomly selected clones were sequenced and a total of 633 sequences were analyzed by bioinformatic programs. The sequences were grouped into 213 clusters, with each cluster being considered to be composed of mRNAs derived from the same gene or closely related genes. About 36% of the mRNA sequences showed significant similarity to known proteins in the non-redundant protein database by the NCBI blastx program and appeared to be coding for functional predicted proteins, whereas the remaining 64% had no similar sequences. Two thirds of the predicted proteins were annotated as basic cellular proteins (housekeeping proteins). Among the functional predicted protein sequences, other than the housekeeping proteins, several protease inhibitors including anticoagulants, two metalloproteases and a potential immunosuppressive protein could be identified. These proteins may play important roles during tick feeding and could be novel anti-tick vaccine candidates.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 67(11), 1127-1131, 2005-11-25

    Japanese Society of Veterinary Science

References:  24

Cited by:  2

Codes

  • NII Article ID (NAID)
    110004049154
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09167250
  • NDL Article ID
    7752729
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE  JASI 
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