Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages

Author(s)

    • Kashino Genro KASHINO Genro
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • Kodama Seiji KODAMA Seiji
    • Radiation Biology Laboratory, Radiation Research Center, Frontier Science Innovation Center, Organization for University-Industry-Government Cooperation, Osaka Prefecture University
    • Suzuki Keiji [他] SUZUKI Keiji
    • Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University
    • WATANABE Masami
    • Laboratory of Radiation Biology, Division of Radiation Life Science, Department of Radiation Life Science and Radiation Medical Science, Kyoto University Research Reactor Institute

Abstract

Werner syndrome (WS) is an autosomal recessive disease characterized by multiple progeroid features. The gene responsible for WS, <i>WRN</i>, is a member of the human RecQ helicase family. WRN is unique among this family, associated with an exonuclease activity. In the present study, we established the human 293-derived cell lines, which expressed exogenously truncated WRN protein, lacking the N-terminal exonuclease domain but having normal helicase activity, and found that they were slightly, but nonetheless significantly, radiosensitive than control cell lines, into which the empty vector had been introduced. The truncated WRN-expressing cells also exhibited increased numbers of micronuclei, chromosome aberrations, and the foci of phosphorylated histone H2AX with X-rays. These results suggested a function of WRN exonuclease activity that is separable from helicase activity and is essential for the repair of radiation-induced DNA damages.<br>

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 46(4), 407-414, 2005-12-28

    Journal of Radiation Research Editorial Committee

References:  29

Cited by:  1

Codes

  • NII Article ID (NAID)
    110004061516
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    04493060
  • NDL Article ID
    7751868
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE  NDL-Digital 
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