全国の犬と猫から回収されたマダニからのリケッチアDNAの検出 Detection of Rickettsial DNA in Ixodid Ticks Recovered from Dogs and Cats in Japan

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Abstract

DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19 prefectures, widespread throughout Japan. Rickettsia canada-like DNA was detected from Haemaphysalis sp. removed from a dog in Fukuoka, and 'Candidatus Rickettsia, tarasevichiae'-like DNA was from Ixodes sp. removed from a dog in Hokkaido.

DNA from ticks recovered from 1137 dogs and 133 cats from all over Japan were examined for <i>Rickettsia</i> infection by citrate synthase gene (<i>gltA</i>)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of <i>Rickettsia</i> <i>japonica</i> or closely related <i>Rickettsia</i> spp. strains were detected from 38 ticks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be <i>Rickettsia helvetica</i> or closely related strains. A total of 29 DNA that showed highest homology with <i>Rickettsia akari</i> or closely related strains were detected in 19 prefectures, widespread throughout Japan. <i>Rickettsia canada</i>-like DNA was detected from <i>Haemaphysalis</i> sp. removed from a dog in Fukuoka, and `<i>Candidatus</i> Rickettsia tarasevichiae'-like DNA was from <i>Ixodes</i> sp. removed from a dog in Hokkaido.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 67(12), 1217-1222, 2005-12-25

    Japanese Society of Veterinary Science

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    110004062319
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09167250
  • NDL Article ID
    7776368
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE 
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