Atomic Force Microscopy of Living Cells (Review Paper)

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  • Ushiki Tatsuo
    Department of Anatomy and Histology, School of Medicine, Faculty of Medicine, Niigata University,<BR> 1 Asahimachi-dori, Niigata 951-8510, Japan
  • Yamamoto Susumu
    Department of Anatomy and Histology, School of Medicine, Faculty of Medicine, Niigata University,<BR> 1 Asahimachi-dori, Niigata 951-8510, Japan
  • Hitomi Jiro
    Department of Anatomy and Histology, School of Medicine, Faculty of Medicine, Niigata University,<BR> 1 Asahimachi-dori, Niigata 951-8510, Japan
  • Ogura Shigeaki
    Department of Internal Medicine, School of Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo 060-863, Japan
  • Umemoto Takeshi
    Takatsuka Unit, Seiko Instruments Inc., 563 Takatsuka-shinden, Matsudo, Chiba 271-2222, Japan
  • Shigeno Masatsugu
    Takatsuka Unit, Seiko Instruments Inc., 563 Takatsuka-shinden, Matsudo, Chiba 271-2222, Japan

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タイトル別名
  • Atomic Force Microscopy of Living Cells.

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This paper is a review of our results of the application of atomic force microscopy (AFM) to the three-dimensional observation of living cells. First, we showed AFM images of living cultured cells in fluid. Contact mode AFM of living cells provided precise information on the shape of cellular processes (such as spike-like processes or lamellipodia) at the cellular margin. The contour of cytoskeletal elements just beneath the cell membrane was also clearly observable on the upper surface of the cells. Secondly, we showed the data on the discrepancy between the AFM images of living cells and fixed cells. These findings were useful for evaluating AFM images of living cells. Finally, we described the time-lapse AFM of living cells. A fluid chamber system enabled us to obtain AFM images of living cells for over 1 h at time intervals of 2–4 min. A series of these AFM images were useful for examining the movements of cellular processes in relation to subcellular cytoskeletal elements. Time-lapse movies produced by sequential AFM images also gave a realistic view of the cellular dynamics.

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