Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model

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Author(s)

    • Lokugamage Kumari
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Kariwa Hiroaki
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Lokugamage Nandadeva
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Iwasa Masahiro
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Hagiya Tomohiro
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Araki Koichi
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Tachi Atsushi
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Mizutani Tetsuya
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
    • Takashima Ikuo
    • Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University

Abstract

The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1 focusforming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50% of the HTN virus inoculated mice survived until 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3x10^6 to 1.3x10^8 FFU/gram [g] tissue), brain (2.1x10^7 to 1.2x10^9 FFU /g tissue), and kidney (2.5x10^5 to 1.6x10^7 FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1 -5.3x10^5 FFU /g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice.

Journal

  • Japanese Journal of Veterinary Research

    Japanese Journal of Veterinary Research 51(3-4), 143-149, 2004-02

    The Graduate School of Veterinary Medicine, Hokkaido University

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