Determination of Short Peptide in a Porphyromonas gingivalis Protein Antigen Recognized by Sera from Periodontitis Patients

DOI Web Site 被引用文献3件 参考文献11件
  • Hayakawa Mitsuo
    Department of Biochemistry, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • Suyama Tsutomu
    Department of Biochemistry, Nihon University School of Dentistry at Matsudo
  • Ema Midori
    Department of Biochemistry, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • Abiko Yoshimitsu
    Department of Biochemistry, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

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Porphyromonas gingivalis has been implicated as a major pathogen in periodontal diseases. We previously succeeded in gene cloning of a 200-kDa membrane protein from P. gingivalis 381. The recombinant protein was immunoreacted by antisera from severe periodontitis patients. In this study, we attempted to identify the 200-kDa antigenic protein (200k-AP) using protein database. The recombinant 200k-AP was highly purified and digested with lysyl endopeptidase, and separated using reverse-phase high-pressure liquid column chromatography. Amino acid sequence of a chromatographic separated short peptide was examined and identified as VTVPENGK. By homology-search in protein database, the 8 amino acid sequence was exactly the same as the HagA which was known as useful immunogen to prevent colonization of P. gingivalis. This findings suggest that 200k-AP may be HagA and useful to develop the immunotherapy againstperiodontitis.

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  • ＩＪＯＭＳ

    ＩＪＯＭＳ 1 (1), 79-81, 2002

    日本大学松戸歯学部 口腔科学研究所

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