STRAIN DIFFERENCES OF THE IMMUNOSTIMULATORY EFFECT OF CPG IN OVA-SENSITIZED MICE
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Immunotherapy using adequate adjuvants to enhance immunogenicity and reduce allergenicity has been ideal for the treatment of allergic disease. Recently, immunostimulatory oligodeoxynucleotides containing CpG motifs have been shown as a strong Th 1 inducing adjuvant and demonstrated that co-administration of antigen results in modulation of allergic Th 2 immune response in murine models. In contrast to the effectiveness, no report have been reported the difference of the immunomodulatory effect between BALB/c and C 57 BL/6 mice. Our investigations were conducted to compare the effectiveness of CpG immunization in Th 2 sensitized those mice. At first, we examined IL-12 p 40 production from nasal lymphocytes of non-immunized mice stimulated with CpG in vitro. We found that nasal lymphocytes from BALB/c mice produced higher levels of IL-12 p 40 than those cells from C 57 BL/6 mice. Next, both strains of mice were sensitized with ovalbumin (OVA) intraperiotoneally and then immunized with CpG. OVA-specific IgE, IgG 1, and IgG 2 a antibody responses, Th cytokines from nasal lymphocytes were investigated. OVA plus CpG co-administration induced Th 1 response and reduced Th 2 response in both strains of mice. However, the immunomodulatory effect of CpG in BALB/c mice was higher than that in C 57 BL/6 mice. These results suggest that the effectiveness of CpG for allergic rhinitis was strain dependent and might be useful to clinical application of CpG based immunotherapy for humans.
- Nagoya medical journal
Nagoya medical journal 47(2), 111-122, 2005-03-28
Nagoya City University