Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O:8 from Water

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  • KOUJITANI Eiko
    Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
  • HORISAKA Tomoko
    Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
  • NOMURA Yoshihiro
    Scleroprotein and Leather Research Institute, Faculty Facilities, Tokyo University of Agriculture and Technology
  • HARA-KUDO Yukiko
    Division of Microbiology, National Institute of Health Sciences
  • OKATANI Alexandre Tomomitsu
    Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
  • IWATA Taketoshi
    Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
  • KUMAGAI Susumu
    Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • HAYASHIDANI Hideki
    Division of Animal Life Science, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology

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  • 免疫磁気ビーズ法と寒天重層法の併用による損傷したYersinia enterocolitica O:8の検出

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Abstract

To develop an effective method to isolate an injured pathogenic Yersinia enterocolitica O:8 organism from environmental samples, we compared the isolation of freeze-injured and non-injured Y. enterocolitica O:8 and found that the isolation was more successful when immuno-magnetic separation (IMS) with anti-Y. enterocolitica O:8 antibody was used. Plating onto cefsulodin-irgasan-novobiocin (CIN) agar and Virulent Yersinia enterocolitica (VYE) agar by means of the agar layer method was found to be effective in isolating the injured cells. The alkali treatment which is generally used for selective detection of Yersinia organism failed to isolate freeze-injured pathogenic Y. enterocolitica O:8 cells. Recovery methods without using the alkali treatment were superior for detecting freeze-injured Y. enterocolitica O:8. Our results demonstrate that the IMS and the agar layer methods should be used to isolate injured pathogenic Yersinia organisms from environmental samples such as water.<br>

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