Molecular Typing of VapA-Positive Rhodococcus equi Isolates from Jeju Native Horses, Korea
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- SON Won-Geun
- Department of Veterinary Medicine, Cheju National University
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- LEE Du-Sik
- Department of Veterinary Medicine, Cheju National University
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- YAMATODA Noriko
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- HATORI Fumiko
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- SHIMIZU Nanako
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- KAKUDA Tsutomu
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- SASAKI Yukako
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- TSUBAKI Shiro
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- TAKAI Shinji
- Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
Bibliographic Information
- Other Title
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- 韓国・済州馬から分離されたVapA陽性Rhodococcus equiの遺伝子型別
- Molecular typing of VapA-positive Rhodococcus equi isolates from Jeju native horses
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Abstract
We recently demonstrated the presence of virulence-associated protein antigen (VapA)-positive Rhodococcus equi in Jeju Island, Korea. These bacteria contained one of two distinct plasmid types, a 90-kb type II plasmid, which has been found in isolates from the native Kiso horses of Japan, and a new variant, a 90-kb type V plasmid. However, the genotypic characters of the VapA-positive R. equi from Jeju native horses and their environments are poorly understood. Ninety-eight isolates from soil samples and 89 isolates from fecal samples were collected from five farms that breed or have bred Jeju native horses, and were tested for the presence of VapA by immunoblotting and PCR. Of the 98 soil isolates and 89 fecal isolates, seven and 13 were VapA-positive R. equi, respectively. In 2003, two Jeju foals died suddenly and were brought to the Faculty of Veterinary Medicine, Cheju National University, for postmortem examination. Pure cultures of R. equi were isolated from the lung lesions of both foals. Of the 16 clinical isolates, 14 were VapA-positive R. equi. Of the 34 VapA-positive clinical and environmental isolates, 16 contained the 90-kb type II plasmid and 18 contained a 90-kb type V plasmid. Pulsed-field gel electrophoresis (PFGE) patterns of the VapA-positive isolates from Jeju horses and Kiso horses, containing the 90-kb type II plasmid, were compared and formed two distinct groups. Furthermore, 18 virulent isolates containing the 90-kb type V plasmid formed two distinct PFGE groups (of 16 and two isolates). These results demonstrate that two virulence plasmid types are widespread in R. equi in Jeju native horses. However, there is little diversity in the PFGE patterns of virulent isolates, suggesting the clonal spread of virulent R. equi. The PFGE pattern of the virulent R. equi isolates from Jeju native horses in Korea is not identical to those of Kiso native horses in Japan.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 68 (3), 249-253, 2006
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681403194112
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- NII Article ID
- 110004675732
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD28XkslKltrs%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 7900379
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- PubMed
- 16598168
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed