培養細胞の増殖におよぼすレーザーの出力とエネルギー密度に関する研究

• 茂呂, 祐利子

• 中川, 敏浩

• 安部, 仁晴

• 齋藤, 勇

• 山本, 茂久

• モロ, ユリコ

• ナカガワ, トシヒロ

• アベ, キミハル

• サイトウ, イサム

• ヤマモト, シゲヒサ

• MORO, Yuriko

• NAKAGAWA, Toshihiro

• AMBE, Kimiharu

• SAITO, Isamu

• YAMAMOTO, Shigehisa

書誌事項

タイトル別名

• A Study of the Laser Power and Energy Densities Affecting Proliferation of Cultured Cells

抄録

P(論文)

Low-power laser irradiation on oral tissue is reported to have healing effects caused by accelerating cell growth. However, the effects of high-power laser irradiation on cell growth are unknown. To investigate the effects of high-power laser irradiation on oral tissue, we examined the cell growth of fibroblasts (Balb/3T3(clone)A31), osteoblasts (MC3T3-E1) and epithelial cells (normal human gingival keratinocytes) after laser irradiation in vitro using MTT assay. Cells were cultured during 1 day following laser irradiation once for 30, 60, 90, 120 and 180 seconds with a diode laser (830±10nm, GaAlAs diode, continuous wave, 400mW or 800mW). Non-irradiation cells were used as the control group. As a result, fibroblasts and epithelial cells showed good cell growth under the irradiation of 3.7mW/cm^2 for 90seconds. The irradiation of 3.7mW/cm^2 for 90seconds and of 6.7mW/cm^2 for 60seconds accelerated the cell growth of osteoblasts. These results suggest that laser irradiation of 3.7mW/cm^2 for 90seconds accelerates cell growth. In addition, power density (mW/cm^2) and irradiation time (seconds) are more closely related to cell growth in vitro than to energy density (J/cm^2).

収録刊行物

• 奥羽大学歯学誌

  奥羽大学歯学誌 30 (4), 271-279, 2003-12-30

  奥羽大学

キーワード

• low-power laser
• high-power laser
• in vitro
• power density
• energy density

詳細情報

• CRID: 1050001337622952448
• NII論文ID: 110004685990
• NII書誌ID: AN1010214X
• Web Site: https://ohu-lib.repo.nii.ac.jp/records/2209
• Web Site: https://search.jamas.or.jp/link/ui/2004127396
• 本文言語コード: ja
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles