Morphological Study of Iodoacetic Acid-Induced Cataract by Intravitreal Injection
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To clarify the mechanism underlying the development of nuclear and posterior subcapsular cataract in the rat lens after exposure of iodoacetic acid (IAA). Medication was chosen intravitreal injection to see a response to the lens of IAA. The 10 mM iodoacetic acid dissolved in the saline solution was injected in the dose of 5 μ1 into the vitreous of Spraque-Dawley rat eye. The lens was intermittently extirpated, and was examined by light and electron microscopy. Evans' blue of tracer was injected into the vitreous and the lens itself, and were observed with a dissecting microscope. Four days after administration, the rat lens caused mild nuclear opacity. After 2 weeks, irregular arrangement of bow configuration, and swelling and liquefaction of lens fibers occurred in the posterior cortex. Many, undifferentiated epithelial cells were seen to migrate towards the posterior polar region. Evans' blue injected into the vitreous stained significantly the equatorial zone of the lens. Evans' blue injected into the equatorial region of the lens revealed that the dye moved linearly to the direction of the lens nucleus. This change induced swelling of the lens fibers with the lens nucleus in the early stage. In the equatorial region, the bow architecture was relatively disturbed. It could be considered that the degeneration of the equator part was closely concerned with the nuclear change. Epithelial degeneration at the equator occurred a posterior movement of spherical cells, and ultimately, the formation of posterior subcapsular cataract.
- Kitasato medicine
Kitasato medicine 31(3), 188-193, 2001-06-30