The application of molecular biological tools to epidemiology of African trypanosomosis.

被引用文献1件
  • ESHITA Y.
    Department of Parasitology, Kurume University School of Medicine
  • MAJIWA Phelix A. O.
    International Livestock Research Institute (ILRI)
  • URAKAWA Toyohiko
    Society for Techno-innovation of Agriculture, Forestry and Fisheries (STAFF) Institute
  • INOUE Noboru
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • HIRUMI Kazuko
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine
  • YANAGI Tetsuo
    Department of Protozoology, The Institute of Tropical Medicine, Nagasaki University
  • YONEDA Yutaka
    Department of Parasitology, Kurume University School of Medicine
  • HARA Tatsuru
    Department of Parasitology, Kurume University School of Medicine
  • HIGUCHI Takafumi
    Department of Parasitology, Kurume University School of Medicine
  • FUKUMA Toshihide
    Department of Parasitology, Kurume University School of Medicine
  • HIRUMI Hiroyuki
    The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine

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Difficulties have often been encountered in the field surveys due to a lack of definitive morphological characters, particularly where mixed infections are expected. To address this problem, some molecular biological techniques such as DNA probe hybridization, restriction fragment length polymorphism (RFLP) analysis, the polymerase chain reaction (PCR), analyses of ribosomal DNA, and pulsed-field gel electrophoresis (PFGE), have been applied to the analysis of field samples collected during epidemiological surveys of African trypanosomosis. Concurrent natural infection of different individual tsetse flies and mammalian hosts with different species of the trypanosomes have been demonstrated, through the use of a combination of specific DNA probe hybridization and the PCR. Molecular karyotypes of Trypanosoma brucei species were analyzed by PFGE in 45-2,000 kb range. There are distinctive differences in intermediate and mini-chromosomes among the strains. We have compared the nucleotide sequences of ribosomal DNAs of the parasites by PCR techniques. From this data new phylogenetic tree can be inferred. It is apparent that these technologies can provide powerful tools for identification and diagnosis of trypanosomes in their hosts and vectors, and for their more accurate phylogenetic classification.

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