ATS腎炎におけるメサンギウム融解後の糸球体再構築

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  • イガク ケンキュウ ジッシュウ キソ ハイゾク レポート ATSジンエン ニ オケル メサンギウム ユウカイ ゴ ノ シキュウタイ サイコウチク
  • Reconstruction of Glomerular Capillary Tufts Following Anti-thymocyte Antibody-induced Mesangiolysis

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To analyze the repair process of glomerular capillary after injury, anti-mesangial cell anti-body-induced glomerulonephritis was induced in the rat. Isolated glomeruli were prepared from the kidneys by sieving method. Using focal laser scanning immunofluorescent microscopy, of which the advantages are able to visualize fluorescent molecules in a single plane of focus, to avoid the risk of artifacts by mechanical sectioning, to record images of serial sections at different depths of samples, and to analyze three-dimensionally, we showed that regeneration of glomerular capillaries seemed to proceed chiefly by angiogenetic branching from the remaining microvas-culatres around the avascular lesions.

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