歯周病関連細菌由来LPSとIL‐1が培養線維芽細胞のフィブロネクチン産生に及ぼす影響について  [in Japanese] Effects of Lipopolysaccharide from Periodontopathic Bacteria and Interleukin-1 on Fibronectin Synthesis in Cultured Human Fibroblasts.  [in Japanese]

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Author(s)

    • 久保 浩二 KUBO Kohji
    • 鹿児島大学歯学部歯科保存学講座 (2) Department of Periodontology, Kagoshima University School of Dentistry
    • 小野 智則 ONO Tomonori
    • 鹿児島大学歯学部歯科保存学講座 (2) Department of Periodontology, Kagoshima University School of Dentistry
    • 和泉 雄一 IZUMI Yuichi
    • 鹿児島大学歯学部歯科保存学講座 (2) Department of Periodontology, Kagoshima University School of Dentistry
    • 末田 武 SUEDA Takeshi
    • 鹿児島大学歯学部歯科保存学講座 (2) Department of Periodontology, Kagoshima University School of Dentistry

Abstract

<I>Porphyromonas gingivalis</I> 381, <I>Preotella intermedia</I> ATCC 25611, 歯周炎患者の口腔内より分離培養した<I>Porphyromonas gingivalis</I> wild typeのlipopolysaccharide (LPS) とinterleukin-1 (IL-1) がヒト培養線維芽細胞のフィブロネクチン代謝にどのような影響を及ぼすかを知るために本研究を行った。線維芽細胞を健康歯肉および炎症歯肉, 健康歯根膜から分離培養しLPSあるいはIL-1, LPSとIL-1と共に48時間培養し, 培養上清中のフィプロネクチンを酵素免疫測定法にて測定した。培養線維芽細胞はLPSあるいはIL-1の刺激にてフィプロネクチンを多く産生することがわかった。またその効果はLPSあるいはIL-1単独刺激よりLPSとIL-1の同時刺激の方がより増加することがわかった。

We studied the effects of lipopolysaccharide LPS) from <I>Porphyromonas gingivalis</I>, <I>Prevotella intermedia</I> and <I>Porphyromonas gingivalis</I> wild type, and interleukin-1 (IL-1) on production of fibronectin in cultured human fibroblasts. Fibroblasts were obtained from healthy gingiva, inflamed gingiva and healthy periodontal ligament. After the cells had reached confluence, they were further incubated with or without LPS (final concentration: 10. μg/m<I>l</I>) and/or IL-1 (final concentraion: 0.5-5. μg/m<I>l</I>) for up to 48 hours. Fibronectin levels in culture media were measured using enzyme linked immunosorbent assay. Fibronectin levels were increased by in fibroblasts stimulation with LPS or IL-1. Fibronectin levels stimulated by LPS+IL-1 were much increased compared to those stimulated by LPS or IL-1 alone. From these findings, it is suggested that LPS and/or IL-1 may promote fibronectin synthesis in fibroblasts of periodontal tissue <I>in vivo</I>.

Journal

  • Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

    Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 34(3), 654-664, 1992

    The Japanese Society of Periodontology

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